Duration of Persistent Atrial Fibrillation Is Associated with Alterations in Human Gut Microbiota and Metabolic Phenotypes
Duration of Persistent Atrial Fibrillation Is Associated with Alterations in Human Gut Microbiota and Metabolic Phenotypes
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持续性心房颤动的持续时间与人类肠道微生物群和代谢表型的改变有关
DOI：
10.1128/msystems.00422-19
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发表时间：
2019-12
期刊：
影响因子：
6.4
通讯作者：
Xinchun Yang
中科院分区：
文献类型：
--
作者：
Kun Zuo;Jing Li;Pan Wang;Ye Liu;Zheng Liu;Xi;ong Yin;Xiaoqing Liu;Xinchun Yang
先前的研究表明，心房颤动与肠道微生物群紊乱有关。然而，房颤不同阶段患者的肠道微生物特征仍然很大程度上未知。我们试图确定肠道微生物群和代谢特征的变化是在房颤期间早期发生并保持稳定还是逐渐发展。我们发现持续性房颤<12个月和持续性房颤>12个月的患者具有肠道菌群失调的大多数共同特征。然而，肠道微生物群和代谢结构发生了一些独特的、渐进性的变化，这些变化可能导致心房颤动的进展。本研究全面描述了短期和长期持续性心房颤动患者的肠道微生物群失调和代谢特征，我们的研究结果可能有助于确定针对肠道微生物群的治疗策略，以在早期阶段治疗心房颤动。摘要 心房颤动 (AF) 已被证明与肠道菌群 (GM) 紊乱有关。控制持续性 AF (psAF) 的根本因素尚不清楚，并且 AF 持续时间和 GM 特征之间的关联仍有待表征。因此，本研究旨在调查 psAF 持续时间短和长的患者中 GM 的生态失调，并阐明 GM 和 psAF 维持之间的关系。基于宏基因组测序和代谢组学分析，我们评估了 12 名 psAF <12 个月 (Pers<12m) 患者、8 名 psAF > 12 个月 (Pers>12m) 患者和 20 名对照者的代谢和 GM 特征。我们发现 Pers<12m 和 Pers>12m 患者的 GM 均受到显着干扰，微生物多样性升高、结构不同且组成不同。尽管Pers<12m和Pers>12m患者与对照组共享大量常见细菌，包括84属和404种，但某些细菌在不同AF持续时间下富集程度不同。此外，在 Pers<12m 和 Pers>12m 组中均检测到肠道微生物功能紊乱和 GM 相关代谢改变。 GM 和代谢物与 psAF 的联系与 GM 生态失调和 AF 持续性导致的宿主代谢途径的相互作用和潜在调节一致。我们的结果表明，Pers<12m 和 Pers>12m 组的患者具有许多常见的 GM 和代谢紊乱特征，这些特征可能发生在疾病早期，而 psAF 持续时间延长与某些独特的改变有关。针对 GM 和微生物代谢物进行早期干预以治疗 AF 患者的预防策略是非常有必要的。重要性 在之前的研究中，心房颤动与肠道菌群紊乱有关。然而，房颤不同阶段患者的肠道微生物特征仍然很大程度上未知。我们试图确定肠道微生物群和代谢特征的变化是在房颤期间早期发生并保持稳定还是逐渐发展。我们发现持续性房颤<12个月和持续性房颤>12个月的患者具有肠道菌群失调的大多数共同特征。然而，肠道微生物群和代谢结构发生了一些独特的、渐进性的变化，这些变化可能导致心房颤动的进展。本研究全面描述了短期和长期持续性心房颤动患者的肠道微生物群失调和代谢特征，我们的研究结果可能有助于确定针对肠道微生物群的治疗策略，以在早期阶段治疗心房颤动。
Atrial fibrillation was associated with a disordered gut microbiota in previous research. However, the gut microbiota signature of patients at different stages of atrial fibrillation remains largely unknown. We sought to determine whether the shift in the gut microbiota and metabolic profiles occurs early and remains stable or develops gradually during atrial fibrillation. We found that patients with persistent atrial fibrillation of <12 months and persistent atrial fibrillation of >12 months shared most of the common features of gut microbiota dysbiosis. However, some distinctive and progressive alterations in the gut microbiota and metabolic structure, which may contribute to the progression of atrial fibrillation, were identified. The present study provides a comprehensive description of the dysbiotic gut microbiota and metabolic profiles in patients of short and long persistent atrial fibrillation, and our findings may help identify therapeutic strategies targeting the gut microbiota to treat atrial fibrillation at an early stage. ABSTRACT Atrial fibrillation (AF) has been shown to be associated with disordered gut microbiota (GM). The underlying factors governing persistent AF (psAF) are not well understood, and the association between AF duration and GM profiles remains to be characterized. Thus, the present study aimed at investigating the dysbiosis of GM in patients with short and long psAF duration and illuminating the relationship between the GM and psAF maintenance. Based on metagenomic sequencing and metabolomic analyses, we assessed the metabolic and GM signature in 12 patients with psAF of <12 months (Pers<12m), eight patients with psAF of >12 months (Pers>12m), and 20 controls. We found that the GM in patients with both Pers<12m and Pers>12m was significantly perturbed, with an elevated microbial diversity, distinct structure, and discrepant composition. Although Pers<12m and Pers>12m patients shared a large number of common bacteria with controls, including 84 genera and 404 species, certain bacteria were differently enriched at different AF durations. Furthermore, disturbance in gut microbial function and GM-linked metabolic alterations were detected in both the Pers<12m and Pers>12m groups. The connection of GM and metabolites with psAF is consistent with interaction and potential modulation of host metabolic pathways due to GM dysbiosis with AF persistence. Our results showed that patients of the Pers<12m and Pers>12m groups shared many common disordered GM and metabolic features, which might occur in early disease, while prolonged psAF duration was related to certain unique alterations. Preventative strategies targeting GM and microbial metabolites for early intervention to treat AF patients are highly warranted. IMPORTANCE Atrial fibrillation was associated with a disordered gut microbiota in previous research. However, the gut microbiota signature of patients at different stages of atrial fibrillation remains largely unknown. We sought to determine whether the shift in the gut microbiota and metabolic profiles occurs early and remains stable or develops gradually during atrial fibrillation. We found that patients with persistent atrial fibrillation of <12 months and persistent atrial fibrillation of >12 months shared most of the common features of gut microbiota dysbiosis. However, some distinctive and progressive alterations in the gut microbiota and metabolic structure, which may contribute to the progression of atrial fibrillation, were identified. The present study provides a comprehensive description of the dysbiotic gut microbiota and metabolic profiles in patients of short and long persistent atrial fibrillation, and our findings may help identify therapeutic strategies targeting the gut microbiota to treat atrial fibrillation at an early stage.
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影响因子：
2.7
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
Yamanishi Y
影响因子：
3.4
作者：
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通讯作者：
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