Innate immune responses of primary murine macrophage-lineage cells and RAW 264.7 cells to ligands of Toll-like receptors 2, 3, and 4.
Innate immune responses of primary murine macrophage-lineage cells and RAW 264.7 cells to ligands of Toll-like receptors 2, 3, and 4.
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DOI：
10.1016/j.cimid.2009.07.001
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发表时间：
2010-09
期刊：
影响因子：
--
通讯作者：
Boons GJ
中科院分区：
文献类型：
--
作者：
Berghaus LJ;Moore JN;Hurley DJ;Vandenplas ML;Fortes BP;Wolfert MA;Boons GJ
虽然已经进行了研究，以表征来自单个解剖部位的巨噬细胞（如肺泡巨噬细胞）或小鼠来源的巨噬细胞系对微生物配体的反应，但很少有研究在表型和功能方面比较这些细胞类型。我们直接比较了三种常用的单核-巨噬细胞系（脾巨噬细胞、骨髓源性巨噬细胞和骨髓源性树突状细胞）原代培养物与小鼠白血病单核-巨噬细胞系RAW 264.7细胞表面标志物的表达和功能反应。我们假设RAW 264.7细胞和原代骨髓源性巨噬细胞在表型上相似，并且对结合toll样受体2、3和4的微生物配体有相似的反应。结果表明，RAW 264.7细胞在细胞表面受体和对微生物配体的反应方面最接近骨髓源性巨噬细胞，这些配体通过toll样受体3和4启动细胞活化。然而，当将RAW 264.7细胞的结果外推到其他原代巨噬细胞谱系的结果时，必须谨慎，主要是因为原代巨噬细胞的表型和功能可能随着持续培养而改变。
Although studies have been performed to characterize responses of macrophages from individual anatomical sites (e.g., alveolar macrophages) or of murine-derived macrophage cell lines to microbial ligands, few studies compare these cell types in terms of phenotype and function. We directly compared the expression of cell surface markers and functional responses of primary cultures of three commonly used cells of monocyte-macrophage lineage (splenic macrophages, bone-marrow derived macrophages, and bone-marrow derived dendritic cells) with those of the murine-leukemic monocyte-macrophage cell line, RAW 264.7. We hypothesized that RAW 264.7 cells and primary bone marrow-derived macrophages would be similar in phenotype and would respond similarly to microbial ligands that bind to either Toll-like receptors 2, 3, and 4. Results indicate that RAW 264.7 cells most closely mimic bone marrow-derived macrophages in terms of cell surface receptors and response to microbial ligands that initiate cellular activation via Toll-like receptors 3 and 4. However, caution must be applied when extrapolating findings obtained with RAW 264.7 cells to those of other primary macrophage-lineage cells, primarily because phenotype and function of the former cells may change with continuous culture.
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影响因子：
2.6
作者：
NOZAWA, RT;YANAKI, N;YOKOTA, T
通讯作者：
YOKOTA, T
DOI：
10.1084/jem.175.6.1697
发表时间：
1992-06-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Lee JD;Kato K;Tobias PS;Kirkland TN;Ulevitch RJ
通讯作者：
Ulevitch RJ
影响因子：
2.2
作者：
Lutz, MB;Kukutsch, N;Schuler, G
通讯作者：
Schuler, G
影响因子：
56.9
作者：
Yamamoto, M;Sato, S;Akira, S
通讯作者：
Akira, S
DOI：
10.1016/j.bbrc.2004.12.184
发表时间：
2005-03-11
影响因子：
3.1
作者：
Hirotani, T;Yamamoto, M;Akira, S
通讯作者：
Akira, S