Modeling interlamellar interactions in angle-ply biologic laminates for annulus fibrosus tissue engineering.
Modeling interlamellar interactions in angle-ply biologic laminates for annulus fibrosus tissue engineering.
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DOI：
10.1007/s10237-011-0288-0
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发表时间：
2011-12
影响因子：
3.5
通讯作者：
Elliott, Dawn M.
中科院分区：
文献类型：
--
作者：
Nerurkar, Nandan L.;Mauck, Robert L.;Elliott, Dawn M.
椎间盘纤维环的机械功能是由其高度有序的细胞外基质的组成和微观结构决定的。最近对由间充质干细胞(MSC)种植的纳米纤维支架形成的工程化角度铺设层板的研究表明，胶原纤维组织成交替排列的平面可能在纤维环组织功能中发挥重要作用。具体地说，这些工程化组织可以通过剪切板层间基质来抵抗拉伸变形，因为胶原层在载荷下进行了不同的重新定位。在本工作中，建立了一个超弹性本构模型来描述层间剪切在增强生物层板拉伸响应中的作用，并将其应用于由MSC种子纳米纤维支架形成的工程环状结构的实验结果。将本构模型应用于三种不同纤维取向的双层膜的单轴拉伸应力-应变数据，得到了表征纤维外基质、纤维和层间剪切作用贡献的材料参数。到体外培养10周时，在铺层角度的解剖范围内，层间剪切占单轴拉伸所产生的总应力的近50%。通过模型参数的大小随培养时间的变化，该模型成功地捕捉到了细胞外基质沉积的功能变化。这项工作说明了工程组织作为进一步了解天然组织结构-功能关系的工具的价值，以及作为开发描述它们的本构模型的试验台的价值。
Mechanical function of the annulus fibrosus of the intervertebral disc is dictated by the composition and microstructure of its highly ordered extracellular matrix. Recent work on engineered angle-ply laminates formed from mesenchymal stem cell (MSC)-seeded nanofibrous scaffolds indicates that the organization of collagen fibers into planes of alternating alignment may play an important role in annulus fibrosus tissue function. Specifically, these engineered tissues can resist tensile deformation through shearing of the interlamellar matrix as layers of collagen differentially reorient under load. In the present work, a hyperelastic constitutive model was developed to describe the role of interlamellar shearing in reinforcing the tensile response of biologic laminates, and was applied to experimental results from engineered annulus constructs formed from MSC-seeded nanofibrous scaffolds. By applying the constitutive model to uniaxial tensile stress–strain data for bilayers with three different fiber orientations, material parameters were generated that characterize the contributions of extrafibrillar matrix, fibers, and interlamellar shearing interactions. By 10 weeks of in vitro culture, interlamellar shearing accounted for nearly 50% of the total stress associated with uniaxial extension in the anatomic range of ply angle. The model successfully captured changes in function with extracellular matrix deposition through variations in the magnitude of model parameters with culture duration. This work illustrates the value of engineered tissues as tools to further our understanding of structure–function relations in native tissues and as a test-bed for the development of constitutive models to describe them.
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影响因子：
3
作者：
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通讯作者：
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发表时间：
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影响因子：
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通讯作者：
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