FAR1 and FAR2 regulate the expression of genes associated with lipid metabolism in the rice blast fungus Magnaporthe oryzae.
FAR1 and FAR2 regulate the expression of genes associated with lipid metabolism in the rice blast fungus Magnaporthe oryzae.
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DOI：
10.1371/journal.pone.0099760
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Talbot NJ
中科院分区：
文献类型：
--
作者：
bin Yusof MT;Kershaw MJ;Soanes DM;Talbot NJ
稻瘟病菌 Magnaporthe oryzae 通过称为附着菌的特殊感染结构引起植物病害。这些圆顶形细胞能够产生巨大的内部压力，使侵入性菌丝能够穿透水稻组织。先前的研究表明，脂质体的动员和随后的脂质代谢是成功附着胞介导的植物感染的必要先决条件，这需要将萌发的孢子和芽管的内容物通过自噬回收到发育中的附着胞。在这里，我们着手鉴定稻瘟菌中脂质代谢的假定调节因子。我们报告了 FAR1 和 FAR2 的鉴定，它们编码转录调节因子 Zn2-Cys6 家族的高度保守成员。我们在米霉中产生了 Δfar1、Δfar2 和 Δfar1Δfar2 双突变体，并表明这些缺失突变体在长链脂肪酸上生长不足。此外，Δfar2突变体也无法在乙酸盐和短链脂肪酸上生长。 FAR1 和 FAR2 对于涉及脂肪酸 β-氧化、乙酰辅酶 A 易位、过氧化物酶体生物发生和响应脂质存在的乙醛酸循环的基因的差异表达是必需的。此外，FAR2 对于乙酰辅酶A 合成相关基因的表达是必需的。有趣的是，Δfar1、Δfar2和Δfar1Δfar2突变体在植物感染期间没有显示出可观察到的脂质体动员的延迟或减少，这表明这些转录调节因子控制真菌对脂质底物的利用，但不控制感染相关形态发生期间细胞内脂质储备的动员。
The rice blast fungus Magnaporthe oryzae causes plant disease via specialised infection structures called appressoria. These dome-shaped cells are able to generate enormous internal pressure, which enables penetration of rice tissue by invasive hyphae. Previous studies have shown that mobilisation of lipid bodies and subsequent lipid metabolism are essential pre-requisites for successful appressorium-mediated plant infection, which requires autophagic recycling of the contents of germinated spores and germ tubes to the developing appressorium. Here, we set out to identify putative regulators of lipid metabolism in the rice blast fungus. We report the identification of FAR1 and FAR2, which encode highly conserved members of the Zn2-Cys6 family of transcriptional regulators. We generated Δfar1, Δfar2 and Δfar1Δfar2 double mutants in M. oryzae and show that these deletion mutants are deficient in growth on long chain fatty acids. In addition, Δfar2 mutants are also unable to grow on acetate and short chain fatty acids. FAR1 and FAR2 are necessary for differential expression of genes involved in fatty acid β-oxidation, acetyl-CoA translocation, peroxisomal biogenesis, and the glyoxylate cycle in response to the presence of lipids. Furthermore, FAR2 is necessary for expression of genes associated with acetyl-CoA synthesis. Interestingly, Δfar1, Δfar2 and Δfar1Δfar2 mutants show no observable delay or reduction in lipid body mobilisation during plant infection, suggesting that these transcriptional regulators control lipid substrate utilization by the fungus but not the mobilisation of intracellular lipid reserves during infection-related morphogenesis.
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影响因子：
--
作者：
Hynes, Michael J.;Murray, Sandra L.;Davis, Meryl A.
通讯作者：
Davis, Meryl A.
影响因子：
10.7
作者：
Castresana, J
通讯作者：
Castresana, J
影响因子：
3.6
作者：
Patkar, Rajesh N.;Ramos-Pamplona, Marilou;Naqvi, Naweed I.
通讯作者：
Naqvi, Naweed I.
影响因子：
64.8
作者：
Gilbert, MJ;Thornton, CR;Talbot, NJ
通讯作者：
Talbot, NJ
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影响因子：
2.5
作者：
Lu, Jian-Ping;Liu, Xiao-Hong;Lin, Fu-Cheng
通讯作者：
Lin, Fu-Cheng