First detection of heartland virus (Bunyaviridae: Phlebovirus) from field collected arthropods.
First detection of heartland virus (Bunyaviridae: Phlebovirus) from field collected arthropods.
复制标题
DOI：
10.4269/ajtmh.13-0209
复制
发表时间：
2013-09
期刊：
影响因子：
--
通讯作者：
Nicholson WL
中科院分区：
文献类型：
--
作者：
Savage HM;Godsey MS;Lambert A;Panella NA;Burkhalter KL;Harmon JR;Lash RR;Ashley DC;Nicholson WL
哈特兰病毒(HRTV)是在美国发现的第一种致病性白喉病毒(科：布尼亚病毒科)，最近从密苏里州的两名农民那里被描述。2012年，我们在包括两名患者的农场在内的12个地点收集了56,428只扁虱，代表3个物种。美洲Amblyomma americanum和Dermacentor varablis几乎占了所有收集到的硬蜱的数量。从患者养殖场和附近的保护区收集的10个由美洲斑潜蝇枯竭若虫组成的池，逆转录-聚合酶链式反应呈阳性，其中8个池产生了活病毒。来自该小片段的非结构蛋白的序列数据表明，壁虱毒株与人类毒株非常相似，≥序列同源性为97.6%。这是第一项从野外采集的节肢动物中分离出HRTV并将扁虱作为潜在媒介的研究。美洲Amblyomma americanum可能在幼虫阶段通过摄食病毒宿主而感染，并在春季和初夏传播给人类，此时若虫丰富并积极寻找宿主。
Heartland virus (HRTV), the first pathogenic Phlebovirus (Family: Bunyaviridae) discovered in the United States, was recently described from two Missouri farmers. In 2012, we collected 56,428 ticks representing three species at 12 sites including both patients' farms. Amblyomma americanum and Dermacentor variabilis accounted for nearly all ticks collected. Ten pools composed of deplete nymphs of A. americanum collected at a patient farm and a nearby conservation area were reverse transcription-polymerase chain reaction positive, and eight pools yielded viable viruses. Sequence data from the nonstructural protein of the Small segment indicates that tick strains and human strains are very similar, ≥ 97.6% sequence identity. This is the first study to isolate HRTV from field-collected arthropods and to implicate ticks as potential vectors. Amblyomma americanum likely becomes infected by feeding on viremic hosts during the larval stage, and transmission to humans occurs during the spring and early summer when nymphs are abundant and actively host seeking.
登录
查看更多内容
影响因子：
2.1
作者：
Keirans, JE;Durden, LA
通讯作者：
Durden, LA
影响因子：
10.7
作者：
TAMURA, K;NEI, M
通讯作者：
NEI, M
影响因子：
2.1
作者：
KEIRANS, JE;LITWAK, TR
通讯作者：
LITWAK, TR
影响因子：
9.4
作者：
Lambert, Amy J.;Lanciotti, Robert S.
通讯作者：
Lanciotti, Robert S.
影响因子：
10.7
作者：
SAITOU, N;NEI, M
通讯作者：
NEI, M