Imaging Flow Cytometry Analysis of CEACAM Binding to Opa-Expressing Neisseria gonorrhoeae.
Imaging Flow Cytometry Analysis of CEACAM Binding to Opa-Expressing Neisseria gonorrhoeae.
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DOI：
10.1002/cyto.a.24037
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发表时间：
2020-10
期刊：
影响因子：
--
通讯作者：
Criss AK
中科院分区：
文献类型：
--
作者：
Werner LM;Palmer A;Smirnov A;Belcher Dufrisne M;Columbus L;Criss AK
Human carcinoembryonic antigen-related cell adhesion molecules (CEACAMs) are a family of receptors that mediate intercellular interactions. Pathogenic bacteria have ligands that bind CEACAMs on human cells. Neisseria gonorrhoeae (Gc) encodes numerous unique outer membrane opacity-associated (Opa) proteins that are ligands for one or more CEACAMs. CEACAMs that are expressed on epithelial cells facilitate Gc colonization, while those expressed on neutrophils affect phagocytosis and consequent intracellular survival of Gc. Since Opa protein expression is phase-variable, variations in receptor tropism affect how individual bacteria within a population interact with host cells. Here we report the development of a rapid, quantitative method for collecting and analyzing fluorescence intensity data from thousands of cells in a population using imaging flow cytometry to detect N-CEACAM bound to the surface of Opa-expressing Gc. We use this method to confirm previous findings regarding Opa-CEACAM interactions and to examine the receptor—ligand interactions of Gc expressing other Opa proteins, as well as for other N-CEACAM proteins.
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影响因子：
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作者：
Dillard JP
通讯作者：
Dillard JP
影响因子：
3.7
作者：
Erdbruegger, Uta;Rudy, Christine K.;Lannigan, Joanne
通讯作者：
Lannigan, Joanne
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Korotkova N;Yang Y;Le Trong I;Cota E;Demeler B;Marchant J;Thomas WE;Stenkamp RE;Moseley SL;Matthews S
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Matthews S
影响因子：
11.4
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GrayOwen, SD;Dehio, C;Meyer, TF
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Meyer, TF
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Martin, Jennifer N.;Ball, Louise M.;Columbus, Linda
通讯作者：
Columbus, Linda