Comparative analysis of dinoflagellate chloroplast genomes reveals rRNA and tRNA genes.
Comparative analysis of dinoflagellate chloroplast genomes reveals rRNA and tRNA genes.
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DOI：
10.1186/1471-2164-7-297
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发表时间：
2006-11-23
期刊：
影响因子：
4.4
通讯作者：
Howe CJ
中科院分区：
文献类型：
--
作者：
Barbrook AC;Santucci N;Plenderleith LJ;Hiller RG;Howe CJ
含有多甲素的甲藻具有高度减少的叶绿体基因组，这与其他含有叶绿体的生物体中发现的不同。基因组减少似乎是基因广泛转移到核基因组的结果。不同寻常的是，被认为保留在叶绿体基因组中的基因是在小的 DNA“小环”上发现的。在这项研究中，我们对来自三种甲藻物种的小环序列组进行了比较。使用 PCR 扩增来自 Amphidinium carterae 的几个小环，以便为 Amphidinium carterae 和 Amphidinium operculatum（两种明显密切相关的含多甲素甲藻）提供一组同源的包含基因的小环。我们比较了这些小环的序列，以确定其叶绿体基因组的内容和特征。我们还与从另一种含甲藻素的甲藻 Heterocapsa triquetra 中获得的小环进行了比较。这些计算机比较揭示了一些在单一物种分析中并不明显的遗传特征。这些特征包括更多的蛋白质编码基因、不寻常的 rRNA 基因（我们显示它们是转录的），以及来自含有多甲素的甲藻叶绿体基因组的 tRNA 基因的第一个例子。小环序列的比较分析使我们能够识别甲藻叶绿体基因组以前未被识别的特征，包括额外的蛋白质和 RNA 基因。叶绿体 rRNA 基因序列与其他生物体中的 rRNA 基因序列完全不同，并且在许多方面类似于一些高度减少的线粒体基因组中发现的 rRNA 基因。甲藻叶绿体基因组中某些 tRNA 基因的保留对叶绿体-线粒体相互作用模型具有重要意义。
Peridinin-containing dinoflagellates have a highly reduced chloroplast genome, which is unlike that found in other chloroplast containing organisms. Genome reduction appears to be the result of extensive transfer of genes to the nuclear genome. Unusually the genes believed to be remaining in the chloroplast genome are found on small DNA 'minicircles'. In this study we present a comparison of sets of minicircle sequences from three dinoflagellate species. PCR was used to amplify several minicircles from Amphidinium carterae so that a homologous set of gene-containing minicircles was available for Amphidinium carterae and Amphidinium operculatum, two apparently closely related peridinin-containing dinoflagellates. We compared the sequences of these minicircles to determine the content and characteristics of their chloroplast genomes. We also made comparisons with minicircles which had been obtained from Heterocapsa triquetra, another peridinin-containing dinoflagellate. These in silico comparisons have revealed several genetic features which were not apparent in single species analyses. The features include further protein coding genes, unusual rRNA genes, which we show are transcribed, and the first examples of tRNA genes from peridinin-containing dinoflagellate chloroplast genomes. Comparative analysis of minicircle sequences has allowed us to identify previously unrecognised features of dinoflagellate chloroplast genomes, including additional protein and RNA genes. The chloroplast rRNA gene sequences are radically different from those in other organisms, and in many ways resemble the rRNA genes found in some highly reduced mitochondrial genomes. The retention of certain tRNA genes in the dinoflagellate chloroplast genome has important implications for models of chloroplast-mitochondrion interaction.
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影响因子：
9.2
作者：
Hackett, JD;Yoon, HS;Bhattacharya, D
通讯作者：
Bhattacharya, D
影响因子：
10.7
作者：
Laatsch, T;Zauner, S;Maier, UG
通讯作者：
Maier, UG
影响因子：
7.4
作者：
Simpson, CL;Stern, DB
通讯作者：
Stern, DB
影响因子：
2.5
作者：
Bachvaroff, TR;Concepcion, GT;Delwiche, CF
通讯作者：
Delwiche, CF
影响因子：
5.6
作者：
Patron, NJ;Waller, RF;Keeling, PJ
通讯作者：
Keeling, PJ