bcl-1 gene rearrangements in mantle cell lymphoma: a comprehensive analysis of 118 cases, including B-5-fixed tissue, by polymerase chain reaction and Southern transfer analysis.
bcl-1 gene rearrangements in mantle cell lymphoma: a comprehensive analysis of 118 cases, including B-5-fixed tissue, by polymerase chain reaction and Southern transfer analysis.
复制标题
套细胞淋巴瘤中的bcl-1基因重排：通过聚合酶链反应和Southern转移分析对118例病例（包括B-5固定组织）进行综合分析。
作者：
R. Chibbar;K. Leung;S. McCormick;K. Ritzkalla;J. Strickler;R. Staggs;S. Poppema;R. Brunning;R. Mcglennen
我们评估了118例套细胞淋巴瘤的聚合酶链反应（PCR）的主要易位簇（MTC）区域和另一个断点对应的探针p94 PS，位于24 kb端粒的MTC位点上的11号染色体。标本包括64个冷冻、19个福尔马林固定、9个B-5固定的淋巴结和26个B-5固定的骨髓活检标本。我们还使用三个单独的bcl-1断裂点探针通过Southern转移分析（SB）分析了来自64个冷冻淋巴结的DNA。用MTC基因座引物和探针在64个冷冻淋巴结中的17个（PCR）和18个（SB）中以及在19个福尔马林固定的淋巴结中的6个、9个B-5固定的淋巴结中的3个和26个B-5固定的骨髓芯中的12个中通过PCR鉴定出基因重排。只有一个病例显示重排与p11 EH探针，对应于位于63 kb端粒的MTC位点的断点。通过PCR或SB未检测到对应于p94 PS探针的断点位点的重排，但我们在约25%的情况下用HinD III消化确定了多态性限制性位点。与其他研究一致，这些结果证实了bcl-1基因座MTC区域的断点紧密聚集，并与30 - 40%的套细胞淋巴瘤相关。bcl-1基因座中的其他断裂点似乎是异质的，并且不能使用现有的探针或引物序列通过PCR或SB检测到。本研究最重要的发现是优化了从B-5固定、石蜡包埋的淋巴结和骨髓活检标本中分离的DNA通过PCR检测免疫球蛋白重链基因重排和MTC区断裂点的方法。从这些组织中获得的结果与从冷冻或福尔马林固定组织中获得的结果相当。
We evaluated 118 cases of mantle cell lymphoma by polymerase chain reaction (PCR) for the major translocation cluster (MTC) region and another breakpoint corresponding to probe p94PS, located 24 kb telomeric to the MTC locus on chromosome 11. The specimens included 64 frozen, 19 formalin-fixed, and 9 B-5-fixed lymph nodes and 26 B-5-fixed bone marrow biopsy specimens. We also analyzed DNA from the 64 frozen lymph nodes by Southern transfer analysis (SB) using three separate bcl-1 breakpoint probes. Gene rearrangements were identified in 17 (PCR) and 18 (SB) of 64 frozen lymph nodes and by PCR in 6 of 19 formalin-fixed lymph nodes, 3 of 9 B-5-fixed lymph nodes, and 12 of 26 B-5-fixed bone marrow cores with MTC locus primers and probe. Only one case showed rearrangement with the p11EH probe that corresponds to breakpoints situated 63 kb telomeric to the MTC locus. No rearrangements were detected by PCR or SB for the breakpoint site corresponding to the p94PS probe, but we identified a polymorphic restriction site with HinD III digest in approximately 25% of the cases. In agreement with other studies, these results confirmed that breakpoints in the MTC region of the bcl-1 locus are tightly clustered and associated with 30 to 40% of mantle cell lymphomas. Other breakpoints in the bcl-1 locus seem to be heterogeneous and cannot be detected by PCR or SB with use of existing probes or primer sequences. The most important finding of our study is optimization of the methodology for the detection of immunoglobulin heavy chain gene rearrangement and MTC region breakpoints by PCR from the DNA isolated from B-5-fixed, paraffin-embedded lymph nodes and bone marrow biopsy specimens. The results obtained from these tissues are comparable to those obtained from frozen or formalin-fixed tissue.
中心细胞淋巴瘤中的染色体 t(11;14)(q13;q32) 断点高度集中于 bcl-1 主要易位簇。
影响因子：
11.4
作者：
Williams,ME;Swerdlow,SH;Meeker,TC
通讯作者：
Meeker,TC
中心细胞淋巴瘤 bcl-1 和 PRAD1 位点处 11 号染色体易位断点的特征。
影响因子：
11.2
作者：
Williams,ME;Swerdlow,SH;Rosenberg,CL;Arnold,A
通讯作者：
Arnold,A
细胞周期蛋白 D1 在具有 11 号染色体 bcl-1 重排的非霍奇金淋巴瘤中过度表达。
DOI：
10.1093/annonc/5.suppl_1.s71
发表时间：
1994
期刊：
Annals of oncology : official journal of the European Society for Medical Oncology
影响因子：
--
作者：
Williams,ME;Swerdlow,SH
通讯作者：
Swerdlow,SH