Aminopeptidase-N from the Helicoverpa armigera (Hubner) brush border membrane vesicles as a receptor of Bacillus thuringiensis crylac delta-endotoxin.
Aminopeptidase-N from the Helicoverpa armigera (Hubner) brush border membrane vesicles as a receptor of Bacillus thuringiensis crylac delta-endotoxin.
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DOI：
10.1007/s002840010297
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发表时间：
2001-10
影响因子：
2.6
通讯作者：
Chhatpar HS
中科院分区：
文献类型：
--
作者：
Ingle SS;Trivedi N;Prasad R;Kuruvilla J;Rao KK;Chhatpar HS
从棉铃虫 2 龄幼虫中制备刷状缘膜囊泡 (BBMV)。免疫印迹显示苏云金芽孢杆菌 (Bt) 毒素的活化 Cry1Ac 的结合。 120 kDa 蛋白被鉴定为 Cry1Ac 型 δ-内毒素的受体。 BBMV 的氨肽酶-N 活性通过 L-亮氨酸对硝基苯胺的水解来测量。比活性为35单位/毫克蛋白质。 BBMV 制剂还显示出低水平的碱性磷酸酶活性。 Zn++ 螯合剂 2,2'-联吡啶和 1,10-菲咯啉在 10 mM 浓度下抑制氨肽酶活性，表明棉铃虫刷状缘中存在锌依赖性氨肽酶。氨肽酶活性随着δ-内毒素浓度的增加而增加。对纯化的 120 kDa 结合蛋白进行 N 末端测序。第一个 10 个氨基酸序列与人类富含半胱氨酸的分泌蛋白 1 前体、抑制素 α 链前体有 60-77% 的相似性。沙门氏菌鞭毛钩蛋白和酵母羧肽酶 S.
Brush border membrane vesicles (BBMVs) were prepared from the 2nd instar larvae of Helicoverpa armigera. Binding of the activated Cry1Ac of Bacillus thuringiensis (Bt) toxin was shown by immunoblot. A 120-kDa protein was identified as a receptor for the Cry1Ac type δ-endotoxin. The aminopeptidase-N activity of BBMVs was measured as the hydrolysis of L-leucine p-nitroanilide. The specific activity was 35 units/mg protein. The BBMV preparation also showed low level of alkaline phosphatase activity. Zn++ chelating agents 2,2′-dipyridyl and 1,10-phenanthroline inhibited aminopeptidase activity at 10 mM concentration, indicating the presence of zinc-dependent aminopeptidase in the brush border of H. armigera. The aminopeptidase activity was increased with increasing concentration of δ-endotoxin. The purified 120-kDa binding protein was N-terminally sequenced. The first 10-amino-acid sequence showed 60–77% similarity with human cysteine-rich secretory protein-1 precursor, inhibin alpha chain precursor. Salmonella flagellar hook protein and yeast carboxypeptidase S.
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DOI：
10.1098/rspb.1991.0084
发表时间：
1991-07-22
影响因子：
4.7
作者：
KNOWLES, BH;KNIGHT, PJK;ELLAR, DJ
通讯作者：
ELLAR, DJ
DOI：
10.1111/j.1432-1033.1989.tb15201.x
发表时间：
1989-12-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
VANRIE, J;JANSENS, S;VANMELLAERT, H
通讯作者：
VANMELLAERT, H
影响因子：
2.8
作者：
CUMMINGS, CE;ELLAR, DJ
通讯作者：
ELLAR, DJ
影响因子：
3.5
作者：
SACCHI, VF;PARENTI, P;WOLFERSBERGER, MG
通讯作者：
WOLFERSBERGER, MG
影响因子：
3.5
作者：
SANCHIS, V;ELLAR, DJ
通讯作者：
ELLAR, DJ