Integrated multi-omics reveals common properties underlying stress granule and P-body formation.
Integrated multi-omics reveals common properties underlying stress granule and P-body formation.
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综合的多词揭示了应力颗粒和p体形成的共同特性。
DOI：
10.1080/15476286.2021.1976986
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发表时间：
2021-11-12
期刊：
影响因子：
4.1
通讯作者：
Grant CM
中科院分区：
文献类型：
--
作者：
Kershaw CJ;Nelson MG;Lui J;Bates CP;Jennings MD;Hubbard SJ;Ashe MP;Grant CM
非膜结合区室，如P体（PB）和应激颗粒（SG）在环境胁迫后的基因表达调控中发挥重要作用。我们系统地和定量地测定了营养胁迫前后形成的PBs和SGs的蛋白质和mRNA组成。我们发现，高分子量（HMW）复合物存在之前，我们建议葡萄糖耗尽，可能作为种子，进一步凝聚的蛋白质形成成熟的PB和SG。我们鉴定了具有低复杂性和RNA结合结构域的蛋白质的富集，以及在营养胁迫后翻译不良的长结构mRNA。PB和SG之间共享许多蛋白质和mRNA，包括在液-液相分离期间促进冷凝物相互作用的几种多价RNA结合蛋白。我们发现了许多常见的蛋白质和RNA组分在PB和SG，支持这些生物浓缩物的成熟过程中的复杂的相互作用概况。这些相互作用网络代表了对应力的可调响应，突出了以前未被认识到的凝析油非均质性。因此，这些研究提供了一个完整的和定量的了解关键的生物冷凝物的动态性质。
Non-membrane-bound compartments such as P-bodies (PBs) and stress granules (SGs) play important roles in the regulation of gene expression following environmental stresses. We have systematically and quantitatively determined the protein and mRNA composition of PBs and SGs formed before and after nutrient stress. We find that high molecular weight (HMW) complexes exist prior to glucose depletion that we propose may act as seeds for further condensation of proteins forming mature PBs and SGs. We identify an enrichment of proteins with low complexity and RNA binding domains, as well as long, structured mRNAs that are poorly translated following nutrient stress. Many proteins and mRNAs are shared between PBs and SGs including several multivalent RNA binding proteins that promote condensate interactions during liquid-liquid phase separation. We uncover numerous common protein and RNA components across PBs and SGs that support a complex interaction profile during the maturation of these biological condensates. These interaction networks represent a tuneable response to stress, highlighting previously unrecognized condensate heterogeneity. These studies therefore provide an integrated and quantitative understanding of the dynamic nature of key biological condensates.
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影响因子：
48
作者：
Huber W;Carey VJ;Gentleman R;Anders S;Carlson M;Carvalho BS;Bravo HC;Davis S;Gatto L;Girke T;Gottardo R;Hahne F;Hansen KD;Irizarry RA;Lawrence M;Love MI;MacDonald J;Obenchain V;Oleś AK;Pagès H;Reyes A;Shannon P;Smyth GK;Tenenbaum D;Waldron L;Morgan M
通讯作者：
Morgan M
影响因子：
12.3
作者：
Castel SE;Levy-Moonshine A;Mohammadi P;Banks E;Lappalainen T
通讯作者：
Lappalainen T
DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
7.8
作者：
Buchan, J. Ross;Muhlrad, Denise;Parker, Roy
通讯作者：
Parker, Roy
影响因子：
64.8
作者：
Hondele, Maria;Sachdev, Ruchika;Weis, Karsten
通讯作者：
Weis, Karsten