Rearrangement and expression of the alpha, beta, and gamma chain T cell receptor genes in human thymic leukemia cells and functional T cells
Rearrangement and expression of the alpha, beta, and gamma chain T cell receptor genes in human thymic leukemia cells and functional T cells
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人胸腺白血病细胞和功能性T细胞中α、β和γ链T细胞受体基因的重排和表达
DOI：
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发表时间：
1986
影响因子：
15.3
通讯作者：
J. Minowada
中科院分区：
文献类型：
--
作者：
J. Minowada
使用代表人类 T 细胞受体基因的 α、β 和 γ 链的 cDNA 和基因组探针，我们检查了代表胸腺分化不同阶段的 14 个人类白血病 T 细胞系和 15 个功能性人类 T 细胞克隆中这些基因的结构和表达。在所有功能性 T 细胞克隆和除一种 (P30/OKUBO) 胸腺白血病细胞系之外的所有细胞系中都发现了 γ 和 β 链基因的重排；所有具有β链重排的细胞系都表达β mRNA。在所有检查的功能性 T 细胞克隆中都发现了 α 链的表达，而使用目前可用的 J 区探针，可以在 13 个功能性克隆中的 10 个中显示出 α 链基因的重排。相比之下，在 10 个白血病 T 细胞系中的 6 个中发现了 α 链的表达，而在这 9 个细胞系中的 6 个中发现了重排。在研究 α 链重排的 14 种白血病细胞系中，有 6 例发现了重排。使用细胞系获得的数据与 T 细胞抗原受体 (TcR) 基因的 γ、β 和 α 链的有序重排和表达一致。本研究中使用的白血病细胞系先前已针对细胞表面抗原和细胞内酶进行了表征。根据这些结果，提出了胸腺发育方案。这些研究确定的发育阶段与本研究中使用分子探针确定的 T 细胞发育阶段并不完全一致。
Using cDNA and genomic probes representing the alpha, beta, and gamma chain of the human T cell receptor genes, we have examined the structure and expression of these genes in 14 human leukemic T cell lines, representing different stages of thymic differentiation, and 15 functional human T cell clones. Rearrangement of the gamma and beta chain genes was found in all of the functional T cell clones and all but one (P30/OKUBO) thymic leukemia cell line; all of the lines that had rearrangement of the beta chain expressed beta mRNA. Expression of the alpha chain was found in all of the functional T cell clones examined, while rearrangement of the alpha chain gene, using currently available probes to the J region, could be shown in 10 of 13 functional clones. In contrast, expression of the alpha chain was found in 6 of 10 leukemic T cell lines, while rearrangement was found in six of these nine cell lines. Of the 14 leukemic cell lines studied for rearrangement of the alpha chain, rearrangement was found in six cases. The data obtained with the cell lines are consistent with an ordered rearrangement and expression of the gamma, beta, and alpha chains of the T cell antigen receptor (TcR) genes. The leukemic cell lines used in the present study have previously been characterized with regard to cell surface antigens and intracellular enzymes. Based on those results a scheme of thymic development was proposed. The developmental stages identified by those studies are not in complete agreement with stages of T cell development, as determined in the present study using molecular probes.
人类 T 细胞伽马链基因：组织、多样性和重排。
DOI：
10.1126/science.3079918
发表时间：
1986
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Quertermous,T;Murre,C;Dialynas,D;Duby,AD;Strominger,JL;Waldman,TA;Seidman,JG
通讯作者：
Seidman,JG