JetValve: Rapid manufacturing of biohybrid scaffolds for biomimetic heart valve replacement.
JetValve: Rapid manufacturing of biohybrid scaffolds for biomimetic heart valve replacement.
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DOI：
10.1016/j.biomaterials.2017.04.033
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发表时间：
2017-07
期刊：
影响因子：
14
通讯作者：
Parker KK
中科院分区：
文献类型：
--
作者：
Capulli AK;Emmert MY;Pasqualini FS;Kehl D;Caliskan E;Lind JU;Sheehy SP;Park SJ;Ahn S;Weber B;Goss JA;Hoerstrup SP;Parker KK
组织工程支架由于其再生潜力而成为心脏瓣膜置换术的一种有前途的解决方案。然而，传统的心脏瓣膜组织工程依赖于资源密集型、基于细胞的制造，这增加了成本并阻碍了临床转化。为了克服这些限制，原位组织工程方法旨在开发引起内源性组织重塑和修复的支架材料和制造工艺。然而，尽管最近在合成材料制造方面取得了进展，但仍然缺乏快速生产仿生心脏瓣膜支架的无细胞自动化方法。在这里，我们设计了一种喷射纺丝工艺，用于快速自动制造纤维心脏瓣膜支架。定制支架的组成、多尺度结构和机械性能以模拟天然小叶纤维化并组装成三维半月瓣结构。我们证明了这些支架参数的可控调节，并在体外显示出初始的生物相容性和功能性。在绵羊模型中，通过经心尖入路将瓣膜微创展开至肺动脉瓣位置，并显示其功能可持续15小时。
Tissue engineered scaffolds have emerged as a promising solution for heart valve replacement because of their potential for regeneration. However, traditional heart valve tissue engineering has relied on resource-intensive, cell-based manufacturing, which increases cost and hinders clinical translation. To overcome these limitations, in situ tissue engineering approaches aim to develop scaffold materials and manufacturing processes that elicit endogenous tissue remodeling and repair. Yet despite recent advances in synthetic materials manufacturing, there remains a lack of cell-free, automated approaches for rapidly producing biomimetic heart valve scaffolds. Here, we designed a jet spinning process for the rapid and automated fabrication of fibrous heart valve scaffolds. The composition, multiscale architecture, and mechanical properties of the scaffolds were tailored to mimic that of the native leaflet fibrosa and assembled into three dimensional, semilunar valve structures. We demonstrated controlled modulation of these scaffold parameters and show initial biocompatibility and functionality in vitro. Valves were minimally-invasively deployed via transapical access to the pulmonary valve position in an ovine model and shown to be functional for 15 hours.
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影响因子：
16.1
作者：
Capulli AK;MacQueen LA;Sheehy SP;Parker KK
通讯作者：
Parker KK
影响因子：
9
作者：
Hockaday LA;Kang KH;Colangelo NW;Cheung PY;Duan B;Malone E;Wu J;Girardi LN;Bonassar LJ;Lipson H;Chu CC;Butcher JT
通讯作者：
Butcher JT
影响因子：
14
作者：
Dijkman, Petra E.;Driessen-Mol, Anita;Baaijens, Frank P. T.
通讯作者：
Baaijens, Frank P. T.
DOI：
10.1002/jbm.a.35450
发表时间：
2015-09
期刊：
Journal of biomedical materials research. Part A
影响因子：
--
作者：
Hobson CM;Amoroso NJ;Amini R;Ungchusri E;Hong Y;D'Amore A;Sacks MS;Wagner WR
通讯作者：
Wagner WR
影响因子：
14
作者：
Hinderer, Svenja;Seifert, Jan;Schenke-Layland, Katja
通讯作者：
Schenke-Layland, Katja