Rapid 3D printing of anatomically accurate and mechanically heterogeneous aortic valve hydrogel scaffolds.
Rapid 3D printing of anatomically accurate and mechanically heterogeneous aortic valve hydrogel scaffolds.
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DOI：
10.1088/1758-5082/4/3/035005
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发表时间：
2012-09
期刊：
影响因子：
9
通讯作者：
Butcher JT
中科院分区：
文献类型：
--
作者：
Hockaday LA;Kang KH;Colangelo NW;Cheung PY;Duan B;Malone E;Wu J;Girardi LN;Bonassar LJ;Lipson H;Chu CC;Butcher JT
主动脉瓣具有复杂的三维（3D）解剖结构和异质性，对于长期有效的生物力学功能至关重要。然而，在从头工程化活组织瓣膜策略中模拟这些是具有挑战性的。我们提出了一种新的同步3D打印/光交联技术，用于快速工程化复杂的异质主动脉瓣支架。内径（ID）为12至22 mm的天然解剖和轴对称主动脉瓣几何形状（根壁和三叶）用补充有藻酸盐的聚乙二醇-二丙烯酸酯（PEG-DA）水凝胶（700或8000 MW）进行3D打印。使用Micro-CT对3D打印几何精度进行量化和比较。猪主动脉瓣间质细胞（PAVIC）接种的支架培养长达21天。结果表明，共混的PEG-DA支架的弹性模量可以达到10倍以上的范围（5.3±0.9至74.6±1.5 kPa）。具有机械对比水凝胶的阀导管的3D打印时间优化为14至45分钟，随导管直径线性增加。较大的打印瓣膜具有更高的形状保真度（22、17和12 mm ID猪瓣膜为93.3±2.6、85.1±2.0和73.3±5.2%;简化瓣膜为89.1±4.0、84.1±5.6和66.6±5.2%）。PAVIC接种的支架在21天内保持接近100%的活力。这些结果表明，具有受控光交联的3D水凝胶打印可以快速制造支持细胞植入的解剖学异质瓣膜导管。
The aortic valve exhibits complex three-dimensional (3D) anatomy and heterogeneity essential for long-term efficient biomechanical function. These are, however, challenging to mimic in de novo engineered living tissue valve strategies. We present a novel simultaneous 3D-printing/photocrosslinking technique for rapidly engineering complex, heterogeneous aortic valve scaffolds. Native anatomic and axisymmetric aortic valve geometries (root wall and tri-leaflets) with 12 to 22 mm inner diameters (ID) were 3D printed with poly-ethylene glycol-diacrylate (PEG-DA) hydrogels (700 or 8000 MW) supplemented with alginate. 3D printing geometric accuracy was quantified and compared using Micro-CT. Porcine aortic valve interstitial cells (PAVIC) seeded scaffolds were cultured for up to 21 days. Results showed that blended PEG-DA scaffolds could achieve over 10-fold range in elastic modulus (5.3±0.9 to 74.6±1.5 kPa). 3D printing times for valve conduits with mechanically contrasting hydrogels were optimized to 14 to 45 minutes, increasing linearly with conduit diameter. Larger printed valves had greater shape fidelity (93.3±2.6, 85.1±2.0, and 73.3±5.2% for 22, 17, and 12 mm ID porcine valves; 89.1±4.0, 84.1±5.6, and 66.6±5.2% for simplified valves). PAVIC seeded scaffolds maintained near 100% viability over 21 days. These results demonstrate that 3D hydrogel printing with controlled photocrosslinking can rapidly fabricate anatomical heterogeneous valve conduits that support cell engraftment.
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影响因子：
14
作者：
Benton, Julie A.;Fairbanks, Benjamin D.;Anseth, Kristi S.
通讯作者：
Anseth, Kristi S.
影响因子：
3.4
作者：
Azadani, Ali N.;Chitsaz, Sam;Tseng, Elaine E.
通讯作者：
Tseng, Elaine E.
影响因子：
4.1
作者：
Ballyns, Jeffery J.;Gleghorn, Jason P.;Bonassar, Lawrence J.
通讯作者：
Bonassar, Lawrence J.
影响因子：
--
作者：
Cohen, Daniel L.;Malone, Evan;Bonassar, Lawrence J.
通讯作者：
Bonassar, Lawrence J.
影响因子：
--
作者：
Benton JA;DeForest CA;Vivekanandan V;Anseth KS
通讯作者：
Anseth KS