Actomyosin stiffens the vertebrate embryo during crucial stages of elongation and neural tube closure.
Actomyosin stiffens the vertebrate embryo during crucial stages of elongation and neural tube closure.
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DOI：
10.1242/dev.026211
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发表时间：
2009-02
期刊：
影响因子：
--
通讯作者：
Davidson LA
中科院分区：
文献类型：
--
作者：
Zhou J;Kim HY;Davidson LA
物理力量驱动早期胚胎内组织的运动。传统的和现代的方法已经被用来推断并在极少数情况下测量胚胎的机械性能以及力的位置和大小。脊椎动物背轴的伸长是脊椎动物早期发育中的一个关键事件，但背部组织在胚胎伸长过程中的机制尚不清楚，这些机制后来支持神经管闭合和中枢神经系统的形成。在脊椎动物中，两栖动物胚胎允许对胚胎组织进行复杂的物理操作，这是测量组织机械性能所必需的。本文测定了非洲爪蛙(Xenopus Laevis)背部分离外植体从原肠形成到神经形成的硬度，发现背部组织从不到20pa到80pa以上。通过反复从这些外植体中取出组织，我们发现近轴体细胞中胚层的硬度几乎是脊索或神经板的两倍，至少是内胚层的20倍。从纤维连接蛋白原纤维组装减少或肌动球蛋白收缩能力中断的外植体的硬度测量表明，控制早期两栖类胚胎组织硬度的是肌球蛋白细胞皮质的状态，而不是纤维连接蛋白的积累。
Physical forces drive the movement of tissues within the early embryo. Classical and modern approaches have been used to infer and in rare cases measure mechanical properties and the location and magnitude of forces within embryos. Elongation of the dorsal axis is a critical event in early vertebrate development yet the mechanics of dorsal tissues in driving embryonic elongation that later support neural tube closure and formation of the central nervous system is not known. Among vertebrates, amphibian embryos allow complex physical manipulation of embryonic tissues that are required to measure the mechanical properties of tissues. In this paper we measure the stiffness of dorsal isolate explants of frog (Xenopus laevis) from gastrulation to neurulation and find dorsal tissues stiffen from less than 20 Pa to over 80 Pa. By iteratively removing tissues from these explants we find paraxial somitic mesoderm is nearly twice as stiff as either the notochord or neural plate and at least 20-fold stiffer than the endoderm. Stiffness measurements from explants with reduced fibronectin fibril assembly or disrupted actomyosin contractility suggest that it is the state of the actomyosin cell cortex rather than accumulating fibronectin that controls tissue stiffness in early amphibian embryos.
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影响因子：
2.7
作者：
Elul, T;Keller, R
通讯作者：
Keller, R
影响因子：
9.2
作者：
Cramer, LP
通讯作者：
Cramer, LP
DOI：
10.1668/0003-1569(2000)040
发表时间：
2000-09-01
期刊：
AMERICAN ZOOLOGIST
影响因子：
--
作者：
Farlow, JO;Gatesy, SM;Robinson, JM
通讯作者：
Robinson, JM
影响因子：
2.5
作者：
Davidson, LA;Keller, R;DeSimone, DW
通讯作者：
DeSimone, DW
影响因子：
9.2
作者：
Davidson, Lance A.;Marsden, Mungo;DeSimone, Douglas W.
通讯作者：
DeSimone, Douglas W.