The Administration of the Synbiotic Lactobacillus bulgaricus 6c3 Strain, Inulin and Fructooligosaccharide Decreases the Concentrations of Indoxyl Sulfate and Kidney Damage in a Rat Model.
The Administration of the Synbiotic Lactobacillus bulgaricus 6c3 Strain, Inulin and Fructooligosaccharide Decreases the Concentrations of Indoxyl Sulfate and Kidney Damage in a Rat Model.
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DOI：
10.3390/toxins13030192
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发表时间：
2021-03-07
期刊：
影响因子：
4.2
通讯作者：
García-Cancino A
中科院分区：
文献类型：
--
作者：
Jerez-Morales A;Merino JS;Díaz-Castillo ST;Smith CT;Fuentealba J;Bernasconi H;Echeverría G;García-Cancino A
硫酸吲哚酚（IS）参与慢性肾脏疾病（CKD）及其心血管并发症的进展。建议减少IS的方法之一是合生元的管理。这项工作旨在寻找能够降低血清IS水平的益生菌菌株，并将其与两种益生元（菊粉和低聚果糖（FOS））混合以产生推定的合生元，并在大鼠CKD模型中进行测试。将两组Sprague-Dawley大鼠肾切除。一组（Lac）在饮用水中接受混合物16周，另一组（Nef）没有。对照组（C）包括假肾切除大鼠。采用高效液相色谱-二极管阵列检测器（HPLC-DAD）测定血清肌酐和IS浓度。光学显微镜和双光子激发显微镜用于研究肾脏和心脏样品。服用合生元的Lac组IS减少了0.8%，而Nef组增加了38.8%。肾脏的组织学分析显示，Lac组的纤维化面积增加了12%，Nef组增加了25%。合生元没有减少心脏纤维化。因此，推定的合生素显示在大鼠模型中具有减少IS和CKD进展的功能，但未观察到心脏保护。
Indoxyl sulfate (IS) is involved in the progression of chronic kidney disease (CKD) and in its cardiovascular complications. One of the approaches proposed to decrease IS is the administration of synbiotics. This work aimed to search for a probiotic strain capable to decrease serum IS levels and mix it with two prebiotics (inulin and fructooligosaccharide (FOS)) to produce a putative synbiotic and test it in a rat CKD model. Two groups of Sprague-Dawley rats were nephrectomized. One group (Lac) received the mixture for 16 weeks in drinking water and the other no (Nef). A control group (C) included sham-nephrectomized rats. Serum creatinine and IS concentrations were measured using high-performance liquid chromatography with diode array detector (HPLC-DAD). Optical microscopy and two-photon excitation microscopy was used to study kidney and heart samples. The Lac group, which received the synbiotic, reduced IS by 0.8% while the Nef group increased it by 38.8%. Histological analysis of kidneys showed that the Lac group increased fibrotic areas by 12% and the Nef group did it by 25%. The synbiotic did not reduce cardiac fibrosis. Therefore, the putative synbiotic showed that function reducing IS and the progression of CKD in a rat model, but no heart protection was observed.
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影响因子：
5.6
作者：
Banos, Amanda de Faria;Borges, Natalia A.;Mafra, Denise
通讯作者：
Mafra, Denise
影响因子：
3
作者：
Nowak, Adriana;Libudzisz, Zdzislawa
通讯作者：
Libudzisz, Zdzislawa
影响因子：
81.5
作者：
Romagnani, Paola;Remuzzi, Giuseppe;Anders, Hans-Joachim
通讯作者：
Anders, Hans-Joachim
微生物组代谢组学揭示了慢性肾病中与甘氨酸结合代谢物和多胺代谢相关的肠道微生物群
影响因子：
8
作者：
Feng, Ya-Long;Cao, Gang;Zhao, Ying-Yong
通讯作者：
Zhao, Ying-Yong
影响因子：
2.4
作者：
Lee, H. M.;Lee, Y.
通讯作者：
Lee, Y.