Exopolysaccharide biosynthesis is important for Mesorhizobium tianshanense: plant host interaction

Exopolysaccharide biosynthesis is important for Mesorhizobium tianshanense: plant host interaction
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胞外多糖生物合成对天山中桑根瘤菌很重要:植物宿主相互作用

DOI:
10.1007/s00203-007-0345-3
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发表时间:
2008-01
影响因子:
2.8
通讯作者:
Zhou, Jing
Zhou, Jing
中科院分区:
生物学4区
文献类型:
--
作者:
Zhu, Jun;Cai, Tao;Wang, Peng;Zhong, Zengtao;Zhou, Jing

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天山中根瘤菌是一种能够以根瘤的形式与甘草建立共生关系的固氮菌。根瘤菌的根瘤形成通常需要各种分泌的碳水化合物。为了研究天山木霉胞外多糖(EPS)的产生和功能,我们利用转座子突变进行了全基因组筛选,以确定与EPS产生相关的基因。我们鉴定了7个突变体,它们显著降低了EPS的产量,并发现了一个参与EPS合成激活的双组分传感器激酶/反应调节系统。与野生型细菌相比,EPS突变体形成的生物膜明显减少,结瘤能力严重降低,表明EPS合成在共生过程中发挥着重要作用。
Mesorhizobium tianshanense is a nitrogen-fixing bacterium that can establish symbiotic associations with Glycyrrhiza uralensis in the form of root nodules. Nodule formation in rhizobia often requires various secreted carbohydrates. To investigate exopolysaccharide (EPS) production and function in M. tianshanense, we performed a genome-wide screen using transposon mutagenesis to identify genes involved in EPS production. We identified seven mutants that produced significantly lower amounts of EPS as well as a two-component sensor kinase/response regulator system that is involved in the activation of EPS synthesis. EPS mutants formed significantly less biofilm and displayed severely reduced nodulation capacity than wild type bacteria, suggesting that EPS synthesis can play important roles in the symbiosis process.
DOI: 10.1099/00221287-143-4-1389
发表时间: 1997-04
期刊: Microbiology
影响因子: 1.5
作者:
Jaroslaw E. Król;A. Skorupska
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