Gibberellin application at pre-bloom in grapevines down-regulates the expressions of VvIAA9 and VvARF7, negative regulators of fruit set initiation, during parthenocarpic fruit development.
Gibberellin application at pre-bloom in grapevines down-regulates the expressions of VvIAA9 and VvARF7, negative regulators of fruit set initiation, during parthenocarpic fruit development.
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DOI：
10.1371/journal.pone.0095634
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Lee HJ
中科院分区：
文献类型：
--
作者：
Jung CJ;Hur YY;Yu HJ;Noh JH;Park KS;Lee HJ
坐果仅在受精后开始，并且主要由赤霉素（GAs）和生长素严格调节。这些激素的应用诱导单性结实，不受精坐果，但这种诱导的分子机制知之甚少。在本研究中，我们已经表明，单性结实的果实诱导的GA与生长素信号的相互作用的结果在开花前的应用。在种子二倍体'Tamnara'葡萄的花序发育过程中，在有或没有GA应用的情况下，对坐果起始的假定负调节因子，包括葡萄属生长素/吲哚-3-乙酸转录因子9（VvIAA 9）、葡萄属生长素响应因子7（VvARF 7）和VvARF 8的转录水平进行监测。在不施用GA的情况下，VvIAA 9、VvARF 7和VvARF 8在盛花前以相对较高的水平表达，但在授粉后表达下降。在盛花前14天（DBF）施用GA后，然而，在授粉前5 DBF，VvIAA 9和VvARF 7的表达水平下降。还通过监测有或没有GA应用的生长素生物合成基因和生长素响应基因的表达模式来分析GA应用对生长素水平或生长素信号传导的影响。生长素生物合成基因葡萄属邻氨基苯甲酸合成酶β亚基（VvASB 1-like）、葡萄属YUCCA 2（VvYUC 2）和VvYUC 6的转录水平不受GA施用的显著改变。而生长素反应基因葡萄属Gretchen Hagen3.2（VvGH3.2）和VvGH3.3的表达则随着GA的施用从2 DBF到盛花期被上调。此外，葡萄属GA信号传导基因VvDELLA在VvIAA 9和VvARF 7下调之前，在12 DBF至7 DBF期间通过GA施加而上调。这些结果表明，VvIAA 9和VvARF 7是负调节座果开始在葡萄，和GA信号与生长素信号通过VvDELLA在葡萄单性结实果实发育过程中整合。
Fruit set is initiated only after fertilization and is tightly regulated primarily by gibberellins (GAs) and auxins. The application of either of these hormones induces parthenocarpy, fruit set without fertilization, but the molecular mechanism underlying this induction is poorly understood. In the present study, we have shown that the parthenocarpic fruits induced by GA application at pre-bloom result from the interaction of GA with auxin signaling. The transcriptional levels of the putative negative regulators of fruit set initiation, including Vitis auxin/indole-3-acetic acid transcription factor 9 (VvIAA9), Vitis auxin response factor 7 (VvARF7), and VvARF8 were monitored during inflorescence development in seeded diploid ‘Tamnara’ grapevines with or without GA application. Without GA application, VvIAA9, VvARF7, and VvARF8 were expressed at a relatively high level before full bloom, but decreased thereafter following pollination. After GA application at 14 days before full bloom (DBF); however, the expression levels of VvIAA9 and VvARF7 declined at 5 DBF prior to pollination. The effects of GA application on auxin levels or auxin signaling were also analyzed by monitoring the expression patterns of auxin biosynthesis genes and auxin-responsive genes with or without GA application. Transcription levels of the auxin biosynthesis genes Vitis anthranilate synthase β subunit (VvASB1-like), Vitis YUCCA2 (VvYUC2), and VvYUC6 were not significantly changed by GA application. However, the expressions of Vitis Gretchen Hagen3.2 (VvGH3.2) and VvGH3.3, auxin-responsive genes, were up-regulated from 2 DBF to full bloom with GA application. Furthermore, the Vitis GA signaling gene, VvDELLA was up-regulated by GA application during 12 DBF to 7 DBF, prior to down-regulation of VvIAA9 and VvARF7. These results suggest that VvIAA9 and VvARF7 are negative regulators of fruit set initiation in grapevines, and GA signaling is integrated with auxin signaling via VvDELLA during parthenocarpic fruit development in grapevines.
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影响因子：
7.4
作者：
Goetz, Marc;Hooper, Lauren C.;Koltunow, Anna M.
通讯作者：
Koltunow, Anna M.
DOI：
10.1111/j.1755-0238.1995.tb00086.x
发表时间：
1995-01-01
影响因子：
2.8
作者：
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通讯作者：
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11.6
作者：
GILLASPY, G;BENDAVID, H;GRUISSEM, W
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10.5
作者：
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影响因子：
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作者：
Boss, PK;Thomas, MR
通讯作者：
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