miR-372-3p promotes preeclampsia progression by regulating twist1.
miR-372-3p promotes preeclampsia progression by regulating twist1.
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miR-372-3p通过调节twist1促进先兆子痫进展
DOI：
10.3892/etm.2022.11659
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发表时间：
2022-12
影响因子：
2.7
通讯作者：
Meng, Tao
中科院分区：
文献类型：
--
作者：
Li, Ziwei;Wang, Jie;Li, Dianting;Chen, Haiying;Meng, Tao
关键词：
子痫前期(PE)是世界范围内常见的妊娠相关疾病。PE的主要特征是滋养层细胞的移行和侵袭。已有报道称，microRNAs(MiRs)在PE中起重要作用。本研究旨在探讨子痫前期的发病机制和治疗靶点。在本研究中，逆转录定量聚合酶链式反应分析显示，在PE患者胎盘组织中miR-372-3p的表达水平上调。值得注意的是，与晚发性PE相比，早发性PE患者miR-372-3p的表达水平显著上调。此外，创伤愈合、Transwell和Western blotting的体外分析表明，miR-372-3p过表达分别抑制HTR-8/SVneo滋养层细胞的迁移、侵袭和上皮-间充质转化(EMT)。生物信息学分析和双荧光素酶报告基因分析表明miR-372-3p是由twist家族的bHLH转录因子1(Twist1)表达的。救援实验发现，miR-372-3p过表达通过下调Twist1的表达抑制滋养层细胞的迁移、侵袭和EMT。综上所述，本研究表明miR-372-3p的高水平可能是PE的发病因素之一，也可能成为治疗PE的新靶点。
Preeclampsia (PE) is a common pregnancy-related disorder worldwide. PE is mainly characterized by the defective migration and invasion of trophoblast cells. MicroRNAs (miRs) have been reported to serve an important role in PE. The purpose of the study was to explore the pathogenesis and therapeutic targets of preeclampsia. In the present study, reverse transcription-quantitative PCR analysis revealed that the expression levels of miR-372-3p were upregulated in placental tissues from patients with PE. Notably, the expression levels of miR-372-3p were significantly upregulated in patients with early-onset PE compared with patients with late-onset PE. Moreover, in vitro analysis using wound healing, Transwell and western blotting assays demonstrated that miR-372-3p overexpression inhibited the migration, invasion and epithelial-mesenchymal transition (EMT) of HTR-8/SVneo trophoblast cells, respectively. Bioinformatics analysis and a dual luciferase reporter assay revealed that miR-372-3p is sponged by twist family bHLH transcription factor 1 (twist1). Rescue experiments found that miR-372-3p overexpression suppressed trophoblast cell migration, invasion and EMT by downregulating the expression of twist1. In conclusion, the present study revealed that high level of miR-372-3p may act as a factor to cause PE and may also be a potential novel therapeutic target for PE.
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DOI：
10.2174/1871520620666200516145830
发表时间：
2021-01-01
影响因子：
2.8
作者：
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影响因子：
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作者：
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通讯作者：
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