Partial characterization of nuclear binding sites for retinol delivered by cellular retinol binding protein.
Partial characterization of nuclear binding sites for retinol delivered by cellular retinol binding protein.
复制标题
细胞视黄醇结合蛋白递送的视黄醇核结合位点的部分表征。
DOI：
10.1016/0003-9861(85)90287-5
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发表时间：
1985
影响因子：
3.9
通讯作者：
Chytil,F
中科院分区：
文献类型：
--
作者：
Liau,G;Ong,DE;Chytil,F
Retinol (vitamin A alcohol), which plays an important role in the differentiation of epithelia, can be transferred to chromatinin vitro. Rat liver chromatin can accept retinol in a specific and saturable manner only when the retinol is presented as a complex with cellular retinol-binding protein (CRBP). A partial characterization of the nuclear components responsible for accepting retinol is reported here. A preparation of solubilized chromatin isolated from liver nuclei was able to accept retinol from its complex with CRBP as described previously for nuclei and chromatin. The binding of retinol to chromatin was noncovalent. However, chromatin prepared from nuclei which were incubated with DNase I or micrococcal nuclease did not accept retinol specifically. Chromatin in the form of mono and dinucleosomes also did not accept retinol. However, treatment of nuclei with RNase did not affect the specific binding of retinol. Furthermore, it has been found that retinol was not transferred to purified double or single stranded DNA. These results are interpreted to indicate that the transfer of retinol to specific nuclear binding sites requires a higher order of chromatin structure than that occurring in nucleosome preparations.
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DOI：
--
发表时间：
1973
期刊：
Biochemical and Biophysical Research Communications - BBRC
影响因子：
--
作者：
D. Hewish;L. Burgoyne
通讯作者：
L. Burgoyne
细胞维生素 A 结合蛋白的免疫化学研究。
影响因子：
5.2
作者：
Ong,DE;Chytil,F
通讯作者：
Chytil,F
HMG 14 和 17 与活跃转录基因的相互作用
影响因子：
64.5
作者：
S. Weisbrod;M. Groudine;H. Weintraub
通讯作者：
H. Weintraub
在染色质和细胞核的有限核酸酶消化物中对 DNA 进行快速电泳分析。
影响因子：
2.9
作者：
F. Creusot;J. Christman
通讯作者：
J. Christman
鉴定从聚丙烯酰胺凝胶转移至硝化纤维的组织特异性核抗原。
DOI：
10.1126/science.7003713
发表时间：
1981
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Glass2nd,WF;Briggs,RC;Hnilica,LS
通讯作者：
Hnilica,LS