The Gut Microbiome of Adults with Allergic Rhinitis Is Characterised by Reduced Diversity and an Altered Abundance of Key Microbial Taxa Compared to Controls
The Gut Microbiome of Adults with Allergic Rhinitis Is Characterised by Reduced Diversity and an Altered Abundance of Key Microbial Taxa Compared to Controls
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与对照组相比，过敏性鼻炎成人肠道微生物组的特点是关键微生物类群的多样性降低和丰度改变
DOI：
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发表时间：
2020
影响因子：
2.8
通讯作者：
A. Cox
中科院分区：
文献类型：
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作者：
Annabelle M. Watts;N. West;Ping Zhang;Peter K. Smith;A. Cripps;A. Cox
简介：独特的肠道微生物定植模式与婴儿过敏性疾病的发病相关;然而，没有足够的证据来确定异常的微生物组成模式是否持续存在于成人过敏性鼻炎（AR）患者中。目的：比较成人AR患者和对照者的肠道微生物组组成。研究方法：通过16S rRNA基因V3-V4高变区的下一代测序，比较了57名成人AR患者（39.06 ± 13.29岁）和23名对照（CG; 36.55 ± 10.51岁）粪便样本中的肠道微生物组成。使用NCBI 16 S rRNA基因序列数据库的基于参考的方法进行分类学分类和身份分配。结果如下：与CG相比，AR队列中通过香农指数确定的物种丰富度显著降低（AR为4.35 ± 0.59，CG为4.65 ± 0.55，p = 0.037）;还注意到操作分类单位（OTU）计数、逆Simpson和CHAO1多样性指数降低的趋势。拟杆菌（p = 0.014）在AR组中比在CG中显著更丰富。相反，AR组中厚壁菌门的丰度显著低于CG组（p = 0.006）。与CG相比，在AR队列中还观察到副拟杆菌丰度增加（p = 0.008）以及草酸盐（p = 0.001）和梭菌目（p = 0.005）丰度减少。结论：成年AR患者具有独特的肠道微生物组特征，与对照组相比，微生物多样性减少，某些微生物的丰度改变。这项研究的结果提供了证据，证明成年AR患者中存在独特的肠道微生物模式，并需要以机制研究的形式进行进一步研究。
Introduction: Unique gut microbial colonisation patterns are associated with the onset of allergic disease in infants; however, there is insufficient evidence to determine if aberrant microbial composition patterns persist in adult allergic rhinitis (AR) sufferers. Objective: To compare the gut microbiome composition between adult AR sufferers and controls. Methods: Gut microbial composition in stool samples was compared between 57 adult AR sufferers (39.06 ± 13.29 years) and 23 controls (CG; 36.55 ± 10.51 years) via next-generation sequencing of the V3–V4 hypervariable regions of the 16S rRNA gene. Taxonomic classification and identity assignment was performed using a reference-based approach with the NCBI database of 16S rRNA gene sequences. Results: Species richness determined via the Shannon index was significantly reduced in the AR cohort compared to the CG (4.35 ± 0.59 in AR vs. 4.65 ± 0.55 in CG, p = 0.037); trends for reductions in operational taxonomic unit (OTU) counts, inverse Simpson, and CHAO1 diversity indices were also noted. Bacteroidetes (p = 0.014) was significantly more abundant in the AR group than in the CG. In contrast, the Firmicutes phylum was significantly less abundant in the AR group than in the CG (p = 0.006). An increased abundance of Parabacteroides (p = 0.008) and a reduced abundance of Oxalobacter (p = 0.001) and Clostridiales (p = 0.005) were also observed in the AR cohort compared to the CG. Conclusion: Adult AR sufferers have a distinct gut microbiome profile, marked by a reduced microbial diversity and altered abundance of certain microbes compared to controls. The results of this study provide evidence that unique gut microbial patterns occur in AR sufferers in adulthood and warrant further examination in the form of mechanistic studies.
DOI：
10.1016/j.jaci.2016.06.003
发表时间：
2016-09
期刊：
The Journal of allergy and clinical immunology
影响因子：
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作者：
Noval Rivas M;Chatila TA
通讯作者：
Chatila TA