Analysis of Myeloid Cells in Mouse Tissues with Flow Cytometry.

Analysis of Myeloid Cells in Mouse Tissues with Flow Cytometry.
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使用流式细胞仪分析小鼠组织中的骨髓细胞

DOI:
10.1016/j.xpro.2020.100029
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发表时间:
2020-06-19
期刊:
影响因子:
--
通讯作者:
Ginhoux F
Ginhoux F
中科院分区:
其他
文献类型:
--
作者:
Liu Z;Gu Y;Shin A;Zhang S;Ginhoux F

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Myeloid cells, including dendritic cells (DCs), granulocytes, monocytes, monocyte-derived cells and macrophages, are important players in the immune response, but their identification is not as clear as lymphocytes, especially in tissues. This protocol details the step-by-step procedure for the analysis of myeloid populations in various mouse tissues by flow cytometry. For complete details on the use and execution of this protocol, please refer to. Preparation of single-cell suspension from mouse tissues Analysis of tissue-resident macrophages in mouse tissues Gating strategy for myeloid cells in mouse organs Myeloid cells, including dendritic cells (DCs), granulocytes, monocytes, monocyte-derived cells and macrophages, are important players in the immune response, but their identification is not as clear as lymphocytes, especially in tissues. This protocol details the step-by-step procedure for the analysis of myeloid populations in various mouse tissues by flow cytometry.
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