Expression of nicotinamide N-methyltransferase in hepatocellular carcinoma is associated with poor prognosis.
Expression of nicotinamide N-methyltransferase in hepatocellular carcinoma is associated with poor prognosis.
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DOI：
10.1186/1756-9966-28-20
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发表时间：
2009-02-16
期刊：
影响因子：
--
通讯作者：
Kim DS
中科院分区：
文献类型：
--
作者：
Kim J;Hong SJ;Lim EK;Yu YS;Kim SW;Roh JH;Do IG;Joh JW;Kim DS
肝细胞癌是成人肝脏中最常见的肿瘤，尽管治疗方法多种多样，但其复发率和死亡率很高。在这项研究中，烟酰胺N-甲基转移酶(NNMT)是药物代谢中的一个关键酶，被认为是一个潜在的预后因素。对120例原发性肝细胞癌患者的冰冻肿瘤和癌旁组织进行了研究。用实时定量逆转录聚合酶链式反应(RT-PCR)检测NNMT和内对照基因的表达。分析NNMT基因表达水平与临床病理参数及临床转归的关系。癌组织中NNMT基因表达水平显著低于癌旁组织(P<；0.0001)，且与肿瘤分期显著相关(P=0.010)。此外，根据肿瘤NNMT mRNA水平对患者进行分层显示，NNMT mRNA表达水平高的患者总生存期(OS)和无病生存期(DFS)均明显缩短(P=0.053)(P=0.016)。多因素分析显示，NNMT表达(P=0.0096)和肿瘤分期(P=0.0017)是影响DFS预后的重要因素。本研究结果表明，NNMT基因的表达与肝细胞癌的分期和无瘤生存时间有关。由于NNMT具有广泛的底物特异性，可以改变化疗的疗效和不良反应，因此NNMT作为一种预后因素在更大的肝癌患者队列中的作用值得进一步研究。
Hepatocellular carcinoma (HCC) is the most common tumor in the adult liver, with high relapse and mortality rates despite diverse treatment modalities. In this study, nicotinamide N-methyltransferase (NNMT), a key enzyme in drug metabolism, was investigated as a potential prognostic factor. Frozen tumors and non-cancerous surrounding tissues from 120 patients with primary HCC were studied. Expressions of NNMT and internal control genes were measured by real-time reverse-transcription PCR (RT-PCR). The relationship of NNMT mRNA level with clinicopathologic parameters and clinical outcome was evaluated. NNMT mRNA level is markedly reduced in HCCs compared to non-cancerous surrounding tissues (P < 0.0001), and NNMT expression in tumors was significantly correlated with tumor stage (P = 0.010). Moreover, stratification of patients based on tumor NNMT mRNA levels revealed that the patients who expressed higher NNMT mRNA levels tended to have a shorter overall survival (OS) time (P = 0.053) and a significantly shorter disease-free survival (DFS) time (P = 0.016). Both NNMT expression (P = 0.0096) and tumor stage (P = 0.0017) were found to be significant prognostic factors for DFS in a multivariate analysis. The results of this study indicated that NNMT gene expression is associated with tumor stage and DFS time in HCC cases. Because of the broad substrate specificity of NNMT, which could alter the efficacy and adverse effects of chemotherapy, NNMT merits further investigation regarding its role as a prognostic factor with a larger cohort of HCC patients.
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DOI：
10.1093/jnen/61.2.111
发表时间：
2002-02-01
影响因子：
3.2
作者：
Parsons, RB;Smith, ML;Ramsden, DB
通讯作者：
Ramsden, DB
影响因子：
25.7
作者：
Llovet, JM;Beaugrand, M
通讯作者：
Beaugrand, M
DOI：
10.1056/nejmoa0804525
发表时间：
2008-11-06
期刊：
The New England journal of medicine
影响因子：
--
作者：
Hoshida Y;Villanueva A;Kobayashi M;Peix J;Chiang DY;Camargo A;Gupta S;Moore J;Wrobel MJ;Lerner J;Reich M;Chan JA;Glickman JN;Ikeda K;Hashimoto M;Watanabe G;Daidone MG;Roayaie S;Schwartz M;Thung S;Salvesen HB;Gabriel S;Mazzaferro V;Bruix J;Friedman SL;Kumada H;Llovet JM;Golub TR
通讯作者：
Golub TR
影响因子：
3.1
作者：
Jang, JS;Cho, HY;Kim, HW
通讯作者：
Kim, HW
DOI：
10.1111/j.1349-7006.1991.tb01793.x
发表时间：
1991-11-01
期刊：
JAPANESE JOURNAL OF CANCER RESEARCH
影响因子：
--
作者：
NAKAGAWA, K;MIYAZAKI, M;FUJIMURA, S
通讯作者：
FUJIMURA, S