Developing genetic reagents to facilitate recovery, analysis, and maintenance of mouse mutations
Developing genetic reagents to facilitate recovery, analysis, and maintenance of mouse mutations
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开发遗传试剂以促进小鼠突变的恢复、分析和维持
DOI：
10.1007/s003350010095
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发表时间：
2000
期刊：
影响因子：
2.5
通讯作者：
E. Rinchik
中科院分区：
文献类型：
--
作者：
E. Rinchik
抽象的。由于小鼠已经成为哺乳动物功能基因组学和复杂系统/通路分析的卓越模型系统，人们对小鼠突变的产生和分析用作这些分析的工具的兴趣已经升级。我在此主张在继续开发适当标记的染色体重排方面进行平行投资，以用作突变恢复、分析和维持杂交的遗传试剂。具体地说，明显标记的间质染色体缺失对于基于半合性的隐匿基因的区域突变筛选是有价值的，它们也可以被用来简化遗传精细图谱，作为基于位置克隆/候选策略的基因鉴定的前奏。显性标记的染色体倒位也表现出某种隐性表型，可以在更广泛的基于纯合性的区域突变筛选中加以利用，对于简化、低成本和减少错误的突变库维护具有重要价值。还讨论了与遗传背景有关的几个问题，特别是从遗传试剂资源开发的角度。
Abstract. Because the mouse has become the pre-eminent model system for functional genomics and analysis of complex-systems/pathways in mammals, there has been an escalation of interest in the generation and analysis of mouse mutations to use as tools in these analyses. I argue here for a parallel investment in continuing the development of appropriately marked chromosomal rearrangements to use as genetic reagents in mutation recovery, analysis, and maintenance crosses. Specifically, visibly marked interstitial chromosomal deletions can be valuable for regional mutagenesis screens for recessives based on hemizygosity, and they can also be used to simplify genetic fine-mapping as a prelude to gene identification based on positional cloning/candidacy strategies. Dominantly marked chromosomal inversions that also manifest some kind of recessive phenotype can be exploited in more extensive regional mutagenesis screens based on homozygosity, and are invaluable for simplified, low-cost and error-reduced mutant-stock maintenance. Also discussed are several issues concerning genetic background, particularly from the point of view of genetic-reagent resource development.
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小鼠7号染色体Fah-Hbb间隔6-11cM亚区的N-乙基-N-亚硝基脲诱变：完成了4557个配子的测试以及31个突变的缺失定位和互补分析。
影响因子：
3.3
作者：
Rinchik,EM;Carpenter,DA
通讯作者：
Carpenter,DA
DOI：
--
发表时间：
1999-04
期刊：
Laboratory animal science
影响因子：
--
作者：
Thomas Doetschman
通讯作者：
Thomas Doetschman
DOI：
10.1016/0168-9525(91)90016-j
发表时间：
1991
期刊：
Trends in genetics : TIG
影响因子：
--
作者：
E. Rinchik
通讯作者：
E. Rinchik
DOI：
10.1073/pnas.95.3.1114
发表时间：
1998-02
影响因子：
11.1
作者：
James W. Thomas;C. LaMantia;T. Magnuson
通讯作者：
James W. Thomas;C. LaMantia;T. Magnuson
小鼠 7 号染色体 pid-Hbb 区域内由 N-乙基-N-亚硝基脲诱导的植入后致死突变定义的四个位点的缺失图谱。
影响因子：
3.3
作者：
Rinchik,EM;Carpenter,DA;Long,CL
通讯作者：
Long,CL