Xenon Protects against Blast-Induced Traumatic Brain Injury in an In Vitro Model.
Xenon Protects against Blast-Induced Traumatic Brain Injury in an In Vitro Model.
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DOI：
10.1089/neu.2017.5360
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发表时间：
2018-04-15
影响因子：
4.2
通讯作者：
Dickinson R
中科院分区：
文献类型：
--
作者：
Campos-Pires R;Koziakova M;Yonis A;Pau A;Macdonald W;Harris K;Edge CJ;Franks NP;Mahoney PF;Dickinson R
本研究的目的是评价惰性气体氙在体外实验室模型中作为爆炸引起的创伤性脑损伤患者治疗的神经保护功效。我们开发了一种新的爆炸性创伤性脑损伤模型，使用C57 BL/6 N小鼠器官型海马脑切片培养暴露于单一冲击波，与由此产生的损伤定量使用碘化丙啶荧光。利用激波管爆炸发生器模拟了以Friedlander波形为模型的爆炸冲击波。爆炸冲击波暴露导致明显的（P < 0.01）损伤，随着冲击波的峰值超压和冲量的增加而增加，并在创伤后72 h表现出继发性损伤。冲击波诱导的碘化丙啶荧光与裂解的caspase-3免疫荧光重叠，表明冲击波诱导的细胞死亡涉及凋亡。与未处理的对照损伤相比，在爆炸暴露后1小时施加的氙（50%atm）在24小时（p < 0.01）、48小时（p < 0.05）和72小时（p < 0.001）后减少损伤。在24 h和72 h，氙处理的损伤切片与未损伤的假手术切片没有显著差异。我们第一次证明，氙气治疗后爆炸创伤性脑损伤减少初始损伤，并防止随后的损伤发展在体外。我们的研究结果支持氙可能是爆炸引起的创伤性脑损伤的潜在一线治疗方法的观点。
The aim of this study was to evaluate the neuroprotective efficacy of the inert gas xenon as a treatment for patients with blast-induced traumatic brain injury in an in vitro laboratory model. We developed a novel blast traumatic brain injury model using C57BL/6N mouse organotypic hippocampal brain-slice cultures exposed to a single shockwave, with the resulting injury quantified using propidium iodide fluorescence. A shock tube blast generator was used to simulate open field explosive blast shockwaves, modeled by the Friedlander waveform. Exposure to blast shockwave resulted in significant (p < 0.01) injury that increased with peak-overpressure and impulse of the shockwave, and which exhibited a secondary injury development up to 72 h after trauma. Blast-induced propidium iodide fluorescence overlapped with cleaved caspase-3 immunofluorescence, indicating that shock-wave–induced cell death involves apoptosis. Xenon (50% atm) applied 1 h after blast exposure reduced injury 24 h (p < 0.01), 48 h (p < 0.05), and 72 h (p < 0.001) later, compared with untreated control injury. Xenon-treated injured slices were not significantly different from uninjured sham slices at 24 h and 72 h. We demonstrate for the first time that xenon treatment after blast traumatic brain injury reduces initial injury and prevents subsequent injury development in vitro. Our findings support the idea that xenon may be a potential first-line treatment for those with blast-induced traumatic brain injury.
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影响因子：
3.4
作者：
Kovesdi E;Kamnaksh A;Wingo D;Ahmed F;Grunberg NE;Long JB;Kasper CE;Agoston DV
通讯作者：
Agoston DV
影响因子：
8.8
作者：
Campos-Pires R;Armstrong SP;Sebastiani A;Luh C;Gruss M;Radyushkin K;Hirnet T;Werner C;Engelhard K;Franks NP;Thal SC;Dickinson R
通讯作者：
Dickinson R
影响因子：
8.8
作者：
Harris, Katie;Armstrong, Scott P.;Dickinson, Robert
通讯作者：
Dickinson, Robert
影响因子：
8.3
作者：
Dingley, J;Tooley, J;Thoresen, M
通讯作者：
Thoresen, M
DOI：
10.1196/annals.1344.025
发表时间：
2005-01-01
期刊：
NEUROPROTECTIVE AGENTS
影响因子：
--
作者：
Abraini, JH;David, HN;Lemaire, M
通讯作者：
Lemaire, M