Conservation and divergence of autonomous pathway genes in the flowering regulatory network of Beta vulgaris.
Conservation and divergence of autonomous pathway genes in the flowering regulatory network of Beta vulgaris.
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DOI：
10.1093/jxb/erq321
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发表时间：
2011-06
影响因子：
6.9
通讯作者：
Müller AE
中科院分区：
文献类型：
--
作者：
Abou-Elwafa SF;Büttner B;Chia T;Schulze-Buxloh G;Hohmann U;Mutasa-Göttgens E;Jung C;Müller AE
从营养生长到生殖发育的转变是一个复杂的过程，需要对多种环境线索和内源信号做出综合反应。在拟南芥中，对春化和长日照具有兼性要求，自主途径的基因通过抑制中央阻遏物和春化调节基因FLC来发挥花启动子的作用。日长季节变化的环境调节受光周期途径及其关键基因CO的控制。核心真双子叶植物石竹科分支中的根叶作物物种Beta vulgaris与拟南芥只有非常远的亲缘关系，是专性长日照植物，包括有或没有春化要求的形式。甜菜中具有相关功能的 FLC 和 CO 同源物已被鉴定，但与春化和光周期途径平行发挥作用的自主途径基因的存在尚未有报道。在这里，这个问题开始通过 RNA 调节基因 FLK 和染色质调节基因 FVE、LD 和 LDL1 的全长同源物的鉴定和遗传图谱来解决。当在拟南芥中过表达时，BvFLK 在 Col-0 背景下加速抽苔，并通过抑制 FLC 完全补充 flk 突变体的晚抽苔表型。相比之下，BvFVE1 的互补分析和甜菜中假定的旁系同源物的存在表明 FVE 同源物的进化分歧。进一步表明，与拟南芥中的 FVE 不同，BvFVE1 受生物钟控制。总之，这些数据为普通拟杆菌自主途径成分的进化保守性提供了第一个证据，同时也表明一个基因的分歧或亚功能化。结果可能具有更广泛的相关性，因为普通双歧杆菌扩大了进化上多样化的物种范围，这些物种受到花转变的差异发育和/或环境调节的影响。
The transition from vegetative growth to reproductive development is a complex process that requires an integrated response to multiple environmental cues and endogenous signals. In Arabidopsis thaliana, which has a facultative requirement for vernalization and long days, the genes of the autonomous pathway function as floral promoters by repressing the central repressor and vernalization-regulatory gene FLC. Environmental regulation by seasonal changes in daylength is under control of the photoperiod pathway and its key gene CO. The root and leaf crop species Beta vulgaris in the caryophyllid clade of core eudicots, which is only very distantly related to Arabidopsis, is an obligate long-day plant and includes forms with or without vernalization requirement. FLC and CO homologues with related functions in beet have been identified, but the presence of autonomous pathway genes which function in parallel to the vernalization and photoperiod pathways has not yet been reported. Here, this begins to be addressed by the identification and genetic mapping of full-length homologues of the RNA-regulatory gene FLK and the chromatin-regulatory genes FVE, LD, and LDL1. When overexpressed in A. thaliana, BvFLK accelerates bolting in the Col-0 background and fully complements the late-bolting phenotype of an flk mutant through repression of FLC. In contrast, complementation analysis of BvFVE1 and the presence of a putative paralogue in beet suggest evolutionary divergence of FVE homologues. It is further shown that BvFVE1, unlike FVE in Arabidopsis, is under circadian clock control. Together, the data provide first evidence for evolutionary conservation of components of the autonomous pathway in B. vulgaris, while also suggesting divergence or subfunctionalization of one gene. The results are likely to be of broader relevance because B. vulgaris expands the spectrum of evolutionarily diverse species which are subject to differential developmental and/or environmental regulation of floral transition.
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影响因子：
5.4
作者：
BOUDRY, P;WIEBER, R;JUNG, C
通讯作者：
JUNG, C
影响因子：
5.4
作者：
El-Mezawy, A;Dreyer, F;Jung, C
通讯作者：
Jung, C
影响因子：
5.3
作者：
Albert VA;Soltis DE;Carlson JE;Farmerie WG;Wall PK;Ilut DC;Solow TM;Mueller LA;Landherr LL;Hu Y;Buzgo M;Kim S;Yoo MJ;Frohlich MW;Perl-Treves R;Schlarbaum SE;Bliss BJ;Zhang X;Tanksley SD;Oppenheimer DG;Soltis PS;Ma H;DePamphilis CW;Leebens-Mack JH
通讯作者：
Leebens-Mack JH
影响因子：
2.6
作者：
Dechyeva, Daryna;Schmidt, Thomas
通讯作者：
Schmidt, Thomas
影响因子：
56.9
作者：
Alonso, JM;Stepanova, AN;Ecker, JR
通讯作者：
Ecker, JR