3D bioprinting dual-factor releasing and gradient-structured constructs ready to implant for anisotropic cartilage regeneration
3D bioprinting dual-factor releasing and gradient-structured constructs ready to implant for anisotropic cartilage regeneration
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3D 生物打印双因子释放和梯度结构结构，可随时植入以进行各向异性软骨再生
DOI：
10.1126/sciadv.aay1422
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发表时间：
2020-09
期刊：
影响因子：
13.6
通讯作者：
Dai Kerong
中科院分区：
文献类型：
--
作者：
Sun Ye;You Yongqing;Jiang Wenbo;Wang Bo;Wu Qiang;Dai Kerong
3D生物打印可以帮助重建你的膝盖。腕关节损伤是非常常见的，并导致关节功能障碍。现有的关节假体不能用宿主关节组织重塑。然而，开发具有结构完整性的大规模仿生各向异性结构模仿天然软骨具有挑战性。在本研究中，我们描述了通过三维（3D）生物打印双因子释放和梯度结构构建的各向异性软骨再生。双因子释放间充质干细胞（MSC）负载水凝胶用于各向异性软骨分化。与赋予机械强度的物理梯度合成生物可降解聚合物一起，3D生物打印的各向异性软骨结构表现出全层完整性，浅层润滑和深层营养供应。体外和体内软骨组织的评价显示组织成熟和组织化可能足以转移到患者体内。总之，一步3D生物打印的双因子释放和梯度结构构建体被生成用于各向异性软骨再生，整合了MSC和3D生物打印治疗受伤或退行性关节的可行性。
3D bioprinting helps to reconstruct your broken knee. Cartilage injury is extremely common and leads to joint dysfunction. Existing joint prostheses do not remodel with host joint tissue. However, developing large-scale biomimetic anisotropic constructs mimicking native cartilage with structural integrity is challenging. In the present study, we describe anisotropic cartilage regeneration by three-dimensional (3D) bioprinting dual-factor releasing and gradient-structured constructs. Dual-factor releasing mesenchymal stem cell (MSC)–laden hydrogels were used for anisotropic chondrogenic differentiation. Together with physically gradient synthetic biodegradable polymers that impart mechanical strength, the 3D bioprinted anisotropic cartilage constructs demonstrated whole-layer integrity, lubrication of superficial layers, and nutrient supply in deep layers. Evaluation of the cartilage tissue in vitro and in vivo showed tissue maturation and organization that may be sufficient for translation to patients. In conclusion, one-step 3D bioprinted dual-factor releasing and gradient-structured constructs were generated for anisotropic cartilage regeneration, integrating the feasibility of MSC- and 3D bioprinting–based therapy for injured or degenerative joints.
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影响因子：
4.8
作者：
Merriam, Aaron R.;Patel, Jay M.;Dunn, Michael G.
通讯作者：
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影响因子：
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作者：
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作者：
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DOI：
10.1073/pnas.1720658115
发表时间：
2018-05-01
影响因子：
11.1
作者：
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通讯作者：
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