Molecular phylogeny of Chinese vegetable mustard (Brassica juncea) based on the internal transcribed spacers (ITS) of nuclear ribosomal DNA
Molecular phylogeny of Chinese vegetable mustard (Brassica juncea) based on the internal transcribed spacers (ITS) of nuclear ribosomal DNA
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基于核糖体DNA内转录间隔区（ITS）的中国芥菜（Brassica juncea）分子系统发育
DOI：
10.1007/s10722-006-9179-0
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发表时间：
2007
影响因子：
2
通讯作者：
Jinghua Yang
中科院分区：
文献类型：
--
作者：
X. Qi;Mingfang Zhang;Jinghua Yang
本研究利用中国蔬菜芜菁（AB基因组）及其亲本芜菁（A基因组）和黑芥（B基因组）的核糖体DNA核内转录间隔区（ITS 1、5.8SrRNA和ITS 2）的序列变异，探讨了这一中国特有的双二倍体物种的分子遗传学和可能的进化模式。本研究共收集了16份中国芥菜材料，其中包括3份B材料。rapa和一份加入材料B。黑鬼结果显示，两个支持度很高的分支，一个包含4份与B基因组物种“B.nigra”亲缘关系较近的芥菜材料，另一个包含12份B材料。juncea和3份A基因组材料。这一分类与叶绿体DNA、线粒体DNA、核DNA限制性片段长度多态性（RFLP）的结果不一致，表明B. juncea与A基因组类型关系密切。对于这种不一致，我们推测B。起源于中国的芥菜类作物经历了二倍体形态类型的不同重组事件，并发生了单向协同进化。传统的B. juncea的结果并不完全支持ITS序列，因此，这些亚种之间的系统发育关系需要在分子水平上重新考虑。
Sequence variation of nuclear internal transcribed spacer regions of ribosomal DNA (ITS1, 5.8S rRNA and ITS2) from Chinese vegetable mustards (AB-genome) and its putative parents Brassica rapa (the A-genome) and Brassica nigra (the B-genome) were used to investigate the molecular phylogeny and the probable evolutional pattern of this amphidiploid species that uniquely formed in China. Totally, 16 accessions of Chinese vegetable mustard those covering nearly all the diverse variations were included in this study, and together with three accessions of B. rapa and one accession of B. nigra. The results disclosed two strongly supported clades, one containing four accessions of vegetable mustard which have closer relationship with B-genome species “B.nigra” lineage and the other containing 12 accessions of B. juncea and three A-genome accessions. This classification was in disagreement with the evidence from chloroplast DNA, mitochondrial DNA, nuclear DNA restriction fragment length polymorphism (RFLP), which suggested that B. juncea was closely related to the A-genome type. For the incongruence, we speculated that the B. juncea crops derived from Chinese have evolved through different recombined events of the diploid morphutypes and evolved unidirectional concerted evolution. The traditional phenotypic classification of B. juncea was not wholly supported by ITS results, and hence the phylogenetic relationships among these subspecies need to be reconsidered on molecular level.
影响因子：
4.1
作者：
Yau-Wen Yang;P. Tai;Y. Chen;Wen-Hsiung Li
通讯作者：
Yau-Wen Yang;P. Tai;Y. Chen;Wen-Hsiung Li
影响因子：
4.1
作者：
Yang, YW;Lai, KN;Li, WH
通讯作者：
Li, WH