Successive Release of Tissue Inhibitors of Metalloproteinase-1 Through Graphene Oxide-Based Delivery System Can Promote Skin Regeneration.
Successive Release of Tissue Inhibitors of Metalloproteinase-1 Through Graphene Oxide-Based Delivery System Can Promote Skin Regeneration.
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通过基于氧化石墨烯的递送系统连续释放金属蛋白酶-1的组织抑制剂可促进皮肤再生
DOI：
10.1186/s11671-017-2305-4
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发表时间：
2017-09-15
影响因子：
--
通讯作者：
Bao Q
中科院分区：
文献类型：
--
作者：
Zhong C;Shi D;Zheng Y;Nelson PJ;Bao Q
The purpose of this study was to testify the hypothesis that graphene oxide (GO) could act as an appropriate vehicle for the release of tissue inhibitors of metalloproteinase-1 (TIMP-1) protein in the context of skin repair. GO characteristics were observed by scanning electron microscopy, atomic force microscopy, and thermal gravimetric analysis. After TIMP-1 absorbing GO, the release profiles of various concentrations of TIMP-1 from GO were compared. GO biocompatibility with fibroblast viability was assessed by measuring cell cycle and apoptosis. In vivo wound healing assays were used to determine the effect of TIMP-1-GO on skin regeneration. The greatest intensity of GO was 1140 nm, and the most intensity volume was 10,674.1 nm (nanometer). TIMP-1 was shown to be continuously released for at least 40 days from GO. The proliferation and viability of rat fibroblasts cultured with TIMP-1-GO were not significantly different as compared with the cells grown in GO or TIMP-1 alone (p> 0.05). Skin defect of rats treated with TIMP-1 and TIMP-1-GO showed significant differences in histological and immunohistochemical scores (p< 0.05). GO can be controlled to release carrier materials. The combination of TIMP-1 and GO promoted the progression of skin tissue regeneration in skin defect.
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影响因子：
3.9
作者：
Cheon, Yeong Ah;Bae, Jun Hyuk;Chung, Bong Geun
通讯作者：
Chung, Bong Geun
影响因子：
1.9
作者：
Urbaniak-Kujda, Donata;Kapelko-Slowik, Katarzyna;Kuliczkowski, Kazimierz
通讯作者：
Kuliczkowski, Kazimierz
影响因子：
3.8
作者：
Vega, E.;Gamisans, F.;Egea, M. A.
通讯作者：
Egea, M. A.
影响因子：
7.4
作者：
Kirkpatrick, Douglas C.;Wightman, R. Mark
通讯作者：
Wightman, R. Mark
影响因子：
5.8
作者：
de Sousa, Irene Pereira;Buttenhauser, Katrin;Bernkop-Schnuerch, Andreas
通讯作者：
Bernkop-Schnuerch, Andreas