Evaluation of antibody-based single cell type imaging techniques coupled to multiplexed imaging of N-glycans and collagen peptides by matrix-assisted laser desorption/ionization mass spectrometry imaging.
Evaluation of antibody-based single cell type imaging techniques coupled to multiplexed imaging of N-glycans and collagen peptides by matrix-assisted laser desorption/ionization mass spectrometry imaging.
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基于抗体的单细胞成像技术与基质辅助激光解吸/电离质谱成像n -聚糖和胶原肽的多重成像技术的评价。
DOI：
10.1007/s00216-023-04983-2
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发表时间：
2023-11
影响因子：
4.3
通讯作者：
Angel, Peggi M.
中科院分区：
文献类型：
--
作者：
Dunne, Jaclyn;Griner, Jake;Romeo, Martin;Macdonald, Jade;Krieg, Carsten;Lim, Mark;Yagnik, Gargey;Rothschild, Kenneth J.;Drake, Richard R.;Mehta, Anand S.;Angel, Peggi M.
关键词：
基质辅助激光解吸/电离质谱成像（MALDI-MSI）与单细胞空间组学方法的整合允许对单细胞空间信息和基质体N-聚糖和细胞外基质蛋白成像进行全面调查。在此，评价了与MALDI-MSI结合的抗体指导的单细胞工作流程的性能。Miralys™可光裂解的质量标记抗体探针（MALDI-IHC，AmberGen，Inc.），GeoMx DSP®（NanoString，Inc.），和成像质谱细胞计数法（IMC，Standard BioTools Inc.）与N-聚糖和细胞外基质肽的MALDI-MSI在福尔马林固定的石蜡包埋组织上连续使用。在MALDI-MSI之前和之后进行单细胞组学方案。数据表明，对于每种模态组合，在同一组织切片上执行两种技术都有一个最佳顺序。总体结论是，MALDI-MSI研究可以在与用于抗体导向的单细胞模式相同的组织切片上完成。这项工作增加了对组织微环境中细胞和细胞外信息的综合利用，以加强对疾病病理起源的研究。在线版本包含补充材料，可通过10.1007/s 00216 -023-04983-2获得。
The integration of matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) with single cell spatial omics methods allows for a comprehensive investigation of single cell spatial information and matrisomal N-glycan and extracellular matrix protein imaging. Here, the performance of the antibody-directed single cell workflows coupled with MALDI-MSI are evaluated. Miralys™ photocleavable mass-tagged antibody probes (MALDI-IHC, AmberGen, Inc.), GeoMx DSP® (NanoString, Inc.), and Imaging Mass Cytometry (IMC, Standard BioTools Inc.) were used in series with MALDI-MSI of N-glycans and extracellular matrix peptides on formalin-fixed paraffin-embedded tissues. Single cell omics protocols were performed before and after MALDI-MSI. The data suggests that for each modality combination, there is an optimal order for performing both techniques on the same tissue section. An overall conclusion is that MALDI-MSI studies may be completed on the same tissue section as used for antibody-directed single cell modalities. This work increases access to combined cellular and extracellular information within the tissue microenvironment to enhance research on the pathological origins of disease. The online version contains supplementary material available at 10.1007/s00216-023-04983-2.
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影响因子：
7.4
作者：
Heijs B;Potthoff A;Soltwisch J;Dreisewerd K
通讯作者：
Dreisewerd K
影响因子：
12.3
作者：
通讯作者：
--
DOI：
10.1021/jasms.1c00189
发表时间：
2021-12-01
影响因子：
3.2
作者：
Clift CL;McLaughlin S;Muñoz M;Suuronen EJ;Rotstein BH;Mehta AS;Drake RR;Alarcon EI;Angel PM
通讯作者：
Angel PM
DOI：
10.1007/978-981-13-2158-0_4
发表时间：
2018-01-01
期刊：
GLYCOBIOPHYSICS
影响因子：
--
作者：
Drake, Richard R.;West, Connor A.;Angel, Peggi M.
通讯作者：
Angel, Peggi M.
DOI：
10.1007/978-1-0716-1593-5_20
发表时间：
2021-01-01
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Clift, Cassandra L;Mehta, Anand;Angel, Peggi M
通讯作者：
Angel, Peggi M