Gene expression pattern Characterization of the two zebra ® sh orthologues of the KAL-1 gene underlying X chromosome-linked Kallmann syndrome
Gene expression pattern Characterization of the two zebra ® sh orthologues of the KAL-1 gene underlying X chromosome-linked Kallmann syndrome
复制标题
基因表达模式 X 染色体连锁卡尔曼综合征背后的 KAL-1 基因的两个 zebra ® 短直系同源物的表征
DOI：
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发表时间：
1999
期刊：
影响因子：
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通讯作者：
Christine Petita
中科院分区：
文献类型：
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作者：
Olivier Ardouina;Renaud Legouisa;Laurent Fasanob;Brigitte David;Henri Kornc;Jean;Christine Petita
The gene underlying X chromosome-linked Kallmann syndrome, KAL-1, has been identi®ed for several years, yet its role in development is still poorly understood. In order to take advantage of the zebra®sh as a model in developmental genetics, we isolated the two KAL-1 orthologues, kal1.1 and kal1.2, in this species. Comparison of deduced protein sequences with the human one shows 75.5 and 66.5% overall homology, respectively. The most conserved domains are the whey acidic protein-like domain and the ®rst of four ®bronectin-like type III repeats. However, kal1.2 putative protein lacks the basic C-terminal domain (20 residues) found in kal1.1 and KAL-1. The expressions of kal1.1 and kal1.2 were studied in the embryo between 6 and 96 hours post fertilization using whole-mount in situ hybridization. Although a few structures express both genes, kal1.1 and kal1.2 expression patterns are largely non-overlapping. Taken together, these patterns match fairly well those previously reported for human KAL-1 and chicken kal1. As regards the olfactory system, kal1.1 is expressed, from 37 h.p.f. onward, in the presumptive olfactory bulbs, whereas kal1.2 transcript is only detected, from 48 h.p.f., in the epithelium of the nasal cavity. The relevance of the zebra®sh as an animal model for studying both the function of KAL-1 in normal development and the developmental failure leading to the olfactory defect in Kallmann syndrome, is discussed. q 2000 Elsevier Science Ireland Ltd. All rights reserved.
DOI：
10.1016/0169-328x(89)90076-4
发表时间：
1989-12-01
期刊：
MOLECULAR BRAIN RESEARCH
影响因子：
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作者：
SCHWANZELFUKUDA, M;BICK, D;PFAFF, DW
通讯作者：
PFAFF, DW