Maternal and offspring high-fat diet leads to platelet hyperactivation in male mice offspring.
Maternal and offspring high-fat diet leads to platelet hyperactivation in male mice offspring.
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母鼠和子代高脂饮食导致雄性小鼠子代血小板过度活化。
DOI：
10.1038/s41598-020-80373-3
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发表时间：
2021-01-14
影响因子：
4.6
通讯作者：
E Hughes C
中科院分区：
文献类型：
--
作者：
Gaspar RS;Unsworth AJ;Al-Dibouni A;Bye AP;Sage T;Stewart M;Wells S;Cox RD;Gibbins JM;Sellayah D;E Hughes C
母亲营养过剩会增加后代患糖尿病和心血管疾病的风险。虽然观察到对心血管健康的显著影响，但尚未研究对血小板生理学的影响。在这里，我们研究了母亲高脂饮食（HF）摄入是否影响精益和肥胖后代的血小板功能。C57 BL 6/N小鼠母鼠在妊娠前和妊娠期间给予HF或对照（C）饮食8周。雄性和雌性后代接受C或HF饮食26周。实验组为：C/C，母鼠和后代饲喂标准实验室饲料; C/HF母鼠饲喂标准实验室饲料，后代饲喂HF饲料; HF/C和HF/HF。进行表型和代谢试验，并采集血液用于血小板研究。与C/C相比，HF组子代肥胖，脂肪堆积，高血脂和胰岛素抵抗。雌性后代没有出现血小板过度活跃，因此我们将重点放在雄性后代上。与C/C相比，HF/HF小鼠的血小板更大、更活跃并呈现氧化应激。母体和后代HF饮食导致雄性小鼠后代血小板过度活化，这表明了一种新的“双重打击”效应。
Maternal over-nutrition increases the risk of diabetes and cardiovascular events in offspring. While prominent effects on cardiovascular health are observed, the impact on platelet physiology has not been studied. Here, we examined whether maternal high-fat diet (HF) ingestion affects the platelet function in lean and obese offspring. C57BL6/N mice dams were given a HF or control (C) diet for 8 weeks before and during pregnancy. Male and female offspring received C or HF diets for 26 weeks. Experimental groups were: C/C, dam and offspring fed standard laboratory diet; C/HF dam fed standard laboratory diet and offspring fed HF diet; HF/C and HF/HF. Phenotypic and metabolic tests were performed and blood collected for platelet studies. Compared to C/C, offspring HF groups were obese, with fat accumulation, hyperglycaemia and insulin resistance. Female offspring did not present platelet hyperactivity, hence we focused on male offspring. Platelets from HF/HF mice were larger, hyperactive and presented oxidative stress when compared to C/C. Maternal and offspring HF diet results in platelet hyperactivation in male mouse offspring, suggesting a novel ‘double-hit’ effect.
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急性高糖性血症诱导血小板多动，而多囊卵巢综合征中胰岛素不会减弱。
影响因子：
5.4
作者：
Aye MM;Kilpatrick ES;Aburima A;Wraith KS;Magwenzi S;Spurgeon B;Rigby AS;Sandeman D;Naseem KM;Atkin SL
通讯作者：
Atkin SL
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影响因子：
11.4
作者：
Qiao J;Arthur JF;Gardiner EE;Andrews RK;Zeng L;Xu K
通讯作者：
Xu K
影响因子：
2.1
作者：
Ghasemi A;Zahediasl S
通讯作者：
Zahediasl S
影响因子：
4.5
作者：
Sousa RML;Ribeiro NLX;Pinto BAS;Sanches JR;da Silva MU;Coêlho CFF;França LM;de Figueiredo Neto JA;Paes AMA
通讯作者：
Paes AMA
影响因子：
--
作者：
OSMOND, C;BARKER, DJP;SIMMONDS, SJ
通讯作者：
SIMMONDS, SJ