Novel epoxy-silica nanoparticles to develop non-enzymatic colorimetric probe for analytical immuno/bioassays.
Novel epoxy-silica nanoparticles to develop non-enzymatic colorimetric probe for analytical immuno/bioassays.
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DOI：
10.1016/j.aca.2018.04.044
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发表时间：
2018-10-22
影响因子：
6.2
通讯作者：
Rusling JF
中科院分区：
文献类型：
--
作者：
Dixit CK;Bhakta S;Macharia J;Furtado J;Suib SL;Rusling JF
我们开发了一种新的方法来在单个锅中制备环氧硅纳米颗粒(EfSiNP)。在不同的制备条件下，环氧官能团的表面覆盖率为150~57000个分子/粒(~1013~1016个分子/毫升200 nm EfSiNPs)。然后，我们通过将品红染料偶联到EfSINP的环氧官能团上，创建了一个红色的探针。将抗鼠免疫球蛋白与品红共固定化，通过功能检测，优化了它们的比例，以获得最佳比例。在99%以上的置信度下，染料与抗体的比值与−为1.00的系数呈良好的负相关。我们使用所开发的非酶比色免疫阳离子探针以直接免疫分析的形式检测小鼠的免疫球蛋白。该方法的灵敏度为427pg/m L。
We have developed a novel method to develop epoxy silica nanoparticles (EfSiNP) in a single pot. High surface coverage of epoxy functional groups between 150 and 57000 molecules per particles (~1013–1016 molecules/mL of 200 nm EfSiNPs) was achieved for different preparation conditions. We then created a red colored probe by conjugating Fuchsin dye to the epoxy functionalities of EfSINPs. Anti-mouse IgG was co-immobilized with Fuchsin and their ratios were optimized for achieving optimum ratios by testing those in functional assays. Dye to antibody ratios were in good negative correlation with a coefficient of −1.00 measured at a confidence level of over 99%. We employed the developed non-enzymatic colorimetric immunonanoprobe for detecting mouse IgG in a direct immunoassay format. We achieved a sensitivity of 427 pg/mL with the assay.
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