Phosphorus removal performance and population structure of phosphorus-accumulating organisms in HA-A/A-MCO sludge reduction process
Phosphorus removal performance and population structure of phosphorus-accumulating organisms in HA-A/A-MCO sludge reduction process
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HA-A/A-MCO污泥减量过程中除磷性能及积磷生物种群结构
DOI：
10.1080/21655979.2016.1197026
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发表时间：
2016-08
期刊：
影响因子：
4.9
通讯作者：
马希钦
中科院分区：
文献类型：
--
作者：
左宁;何进朝;马希钦
摘要我们开发了一种新的污泥减量HA-A/A-MCO(水解-酸化-厌氧/缺氧-多级连续好氧池)工艺，该工艺提高了对磷和氮的去除。其生物处理单元采用A2/O水解酸化、多级连续曝气、连续流除磷工艺，结合出水厌氧含磷废水进行侧流除磷。该工艺具有除磷脱氮与污泥减量同步的特点。性能的改善与聚磷菌(PAO)的种群结构密切相关。本研究探讨了聚磷菌的除磷性能与种群结构的关系。结果表明，当进水磷浓度为8∼12 mg/L时，HA-A/A-MCO工艺的出水磷平均含量仅为0.44 mg/L，达到了GB18918-2002 A级标准。聚磷菌能有效地释放1 mg磷和吸收2.8 mg磷。由于好氧吸收过程中磷的减少，系统通过排放厌氧富磷废水来去除磷。这降低了PAO对过量磷吸收能力的需求。采用细菌纯培养法分离出5株具有典型吸磷除磷特性的聚磷菌。16SrDNA扩增和序列分析表明，不动杆菌属。Ampropedia sp在厌氧释磷过程中起主导作用。此外，德沃西亚属(Devosia sp.)Bdellovibrio sp是好氧池中的主要菌株，也是吸收磷的主要菌株。在缺氧池中检测到未培养的细菌和其他未培养的菌株。
ABSTRACT We developed a new sludge reduction HA-A/A-MCO (Hydrolysis-Acidogenosis-Anaerobic/Anoxic -Multistep Continuous Oxic tank) process, which has improved phosphate (P) and nitrogen (N) removal. Its biological treatment unit uses an A2/O P & N removal process with hydrolysis acidification, multistep continuous aeration, and continuous flow, coupled with sidestream P removal by draining out anaerobic P-bearing wastewater. The process has advanced synchronization of P and N removal and sludge reduction. The improved performance is closely associated with the population structure of P-accumulating organisms (PAOs). This study investigated the relationship between P removal performance and the population structure of PAOs. The results show that the average effluent P content of HA-A/A-MCO process was only 0.44 mg/L, when the influent P concentration was 8∼12 mg/L. The effluent met the A standard set by GB18918-2002. PAOs were able to effectively release 1 mg of P and absorb 2.8 mg of P. The system removed P by draining out anaerobic P-rich wastewater, as P had been reduced in the aerobic absorption process. This reduced the need for excess P uptake ability of the PAOs. The bacterial pure culture method was applied to isolate 5 PAOs with typical P absorption and removel features. 16SrDNA amplification and sequence analysis revealed that Acinetobacter sp. and Lampropedia sp played dominant roles in anaerobic P-releasing process. Moreover, Devosia sp. and Bdellovibrio sp were the primary strains in the aerobic tank, and, they were the major stains for P absorption. Uncultured Bacterium and other uncultured strains were detected in the anoxic tank.
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DOI：
--
发表时间：
2016
期刊：
--
影响因子：
--
作者：
G. Rhee;G. W. Fuhs
通讯作者：
G. Rhee;G. W. Fuhs
DOI：
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发表时间：
2000
期刊：
China Water & Wastewater
影响因子：
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作者：
Z. Bo
通讯作者：
Z. Bo
影响因子：
12.8
作者：
L. Stante;C. M. Cellamare;F. Malaspina;G. Bortone;A. Tilche
通讯作者：
L. Stante;C. M. Cellamare;F. Malaspina;G. Bortone;A. Tilche
DOI：
--
发表时间：
2009
期刊：
China Water & Wastewater
影响因子：
--
作者：
Zong Shu-an
通讯作者：
Zong Shu-an
DOI：
10.1128/mr.59.1.143-169.1995
发表时间：
1995-03
期刊：
Microbiological Reviews
影响因子：
--
作者：
R. Amann;W. Ludwig;K. Schleifer
通讯作者：
R. Amann;W. Ludwig;K. Schleifer