Cord blood Lin(-)CD45(-) embryonic-like stem cells are a heterogeneous population that lack self-renewal capacity.
Cord blood Lin(-)CD45(-) embryonic-like stem cells are a heterogeneous population that lack self-renewal capacity.
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DOI：
10.1371/journal.pone.0067968
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Madrigal A
中科院分区：
文献类型：
--
作者：
Alvarez-Gonzalez C;Duggleby R;Vagaska B;Querol S;Gomez SG;Ferretti P;Madrigal A
人脐血不仅含有造血干细胞，还含有一种罕见的多能胚胎样干细胞群，其造血标志物(LIN、−、CD45、−)阴性，表达典型的多能细胞标志物。这项工作的目的是从人脐血中分离、鉴定和扩大这种ELSC组分，因为它可能为再生医学应用提供一个有价值的细胞来源。我们发现，我们确实可以分离出LIN−CD45CXCR4−群体，该群体具有高核质比的小细胞(直径3-10微米)，表达干细胞标记CD34和CXCR4。然而，与之前的一些报道不同的是，这一部分CD133不呈阳性。此外，尽管这些细胞表达典型的多能细胞的转录本，如SOX2、Oct3/4和NANOG，但它们不能在我们测试的任何支持干细胞生长的培养基中增殖。用流式细胞仪进一步分析LIN−CD45CXCR4的−群体，发现LIN−CD45Nestin+群体也表达CD34(20%)，而CXCR4则为阴性。这些数据表明，脐带血中存在的LIN−CD45−干细胞组分代表了一个具有干细胞表型特征的小的异质性群体，包括以前没有描述的LIN−CD45−Nestn+群体。这项研究还表明，LIN−CD45hUCB−细胞组分的异质性可能是不同群体描述的人脐血细胞群体差异的可能解释，这些群体使用不同的方法分离。由于这些群体的表型与胚胎干细胞相似，因此被广泛称为“类胚胎干细胞”。然而，它们似乎不能自我更新的事实让人们对它们的身份产生了一些怀疑，并警告说，在这个阶段不要将它们定义为“胚胎样干细胞”。
Human umbilical cord blood (hUCB) has been proposed to contain not only haematopoietic stem cells, but also a rare pluripotent embryonic-like stem cell (ELSc) population that is negative for hematopoietic markers (Lin−CD45−) and expresses markers typical of pluripotent cells. The aim of this work was to isolate, characterise and expand this ELSc fraction from hUCB, as it may provide a valuable cell source for regenerative medicine applications. We found that we could indeed isolate a Lin−CD45− population of small cells (3–10 µm diameter) with a high nucleus to cytoplasm ratio that expressed the stem cell markers CD34 and CXCR4. However, in contrast to some previous reports, this fraction was not positive for CD133. Furthermore, although these cells expressed transcripts typical of pluripotent cells, such as SOX2, OCT3/4, and NANOG, they were not able to proliferate in any of the culture media known to support stem cell growth that we tested. Further analysis of the Lin−CD45− population by flow cytometry showed the presence of a Lin−CD45−Nestin+ population that were also positive for CD34 (20%) but negative for CXCR4. These data suggest that the Lin−CD45− stem cell fraction present in the cord blood represents a small heterogeneous population with phenotypic characteristics of stem cells, including a Lin−CD45−Nestin+ population not previously described. This study also suggests that heterogeneity within the Lin−CD45− cell fraction is the likely explanation for differences in the hUCB cell populations described by different groups that were isolated using different methods. These populations have been widely called “embryonic-like stem cell” on the basis of their phenotypical similarity to embryonic stem cells. However, the fact they do not seem to be able to self-renew casts some doubt on their identity, and warns against defining them as “embryonic-like stem cell” at this stage.
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影响因子：
3.7
作者：
Danova-Alt R;Heider A;Egger D;Cross M;Alt R
通讯作者：
Alt R
影响因子：
20.3
作者：
Beltrami, Antonio R.;Cesselli, Daniela;Beltrami, Carlo A.
通讯作者：
Beltrami, Carlo A.
影响因子：
2.9
作者：
Duggleby, Richard C.;Querol, Sergio;Madrigal, J. Alejandro
通讯作者：
Madrigal, J. Alejandro
DOI：
10.1084/jem.20040440
发表时间：
2004-07-19
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Kögler G;Sensken S;Airey JA;Trapp T;Müschen M;Feldhahn N;Liedtke S;Sorg RV;Fischer J;Rosenbaum C;Greschat S;Knipper A;Bender J;Degistirici O;Gao J;Caplan AI;Colletti EJ;Almeida-Porada G;Müller HW;Zanjani E;Wernet P
通讯作者：
Wernet P
影响因子：
4
作者：
D'Ippolito, G;Diabira, S;Schiller, PC
通讯作者：
Schiller, PC