Very small embryonic-like stem cells purified from umbilical cord blood lack stem cell characteristics.
Very small embryonic-like stem cells purified from umbilical cord blood lack stem cell characteristics.
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DOI：
10.1371/journal.pone.0034899
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Alt R
中科院分区：
文献类型：
--
作者：
Danova-Alt R;Heider A;Egger D;Cross M;Alt R
非常小的胚胎样（VSEL）细胞已被描述为存在于鼠骨髓和人脐带血（hUCB）中的骨髓多能干细胞，并且因此对于再生医学具有高度潜在的意义。然而，仍然存在一些关于VSEL细胞，特别是衍生自hUCB的那些细胞的精确身份和性质的问题。出于这个原因，我们已经进行了大量的UCB样品的VSEL细胞的纯化群体的广泛表征。与先前的报道一致，我们发现VSEL细胞是CXCR 4+，具有高密度，确实比HSC小得多，并且具有极高的核/胞质比。它们的核质是非结构化的，并且用Hoechst 33342强烈染色。对胚胎、间充质、神经元或造血干细胞特征性表面标志物的全面FACS筛选显示VSEL细胞上的表达可忽略不计。这些细胞未能在已知支持胚胎或成体干细胞类型的广泛培养条件下在体外扩增，并且微阵列分析揭示了VSEL细胞的转录谱与明确定义的多能和成体干细胞群体以及成熟的造血谱系明显不同。最后，我们通过荧光原位杂交在大多数纯化的VSEL细胞中检测到非整倍体核型。这些数据既不支持胚胎干细胞样表型，也不支持成体干细胞样表型，而是表明hUCB VSEL细胞是与鼠VSEL细胞不可比较的异常和无活性群体。
Very small embryonic-like (VSEL) cells have been described as putatively pluripotent stem cells present in murine bone marrow and human umbilical cord blood (hUCB) and as such are of high potential interest for regenerative medicine. However, there remain some questions concerning the precise identity and properties of VSEL cells, particularly those derived from hUCB. For this reason, we have carried out an extensive characterisation of purified populations of VSEL cells from a large number of UCB samples. Consistent with a previous report, we find that VSEL cells are CXCR4+, have a high density, are indeed significantly smaller than HSC and have an extremely high nuclear/cytoplasmic ratio. Their nucleoplasm is unstructured and stains strongly with Hoechst 33342. A comprehensive FACS screen for surface markers characteristic of embryonic, mesenchymal, neuronal or hematopoietic stem cells revealed negligible expression on VSEL cells. These cells failed to expand in vitro under a wide range of culture conditions known to support embryonic or adult stem cell types and a microarray analysis revealed the transcriptional profile of VSEL cells to be clearly distinct both from well-defined populations of pluripotent and adult stem cells and from the mature hematopoietic lineages. Finally, we detected an aneuploid karyotype in the majority of purified VSEL cells by fluorescence in situ hybridisation. These data support neither an embryonic nor an adult stem cell like phenotype, suggesting rather that hUCB VSEL cells are an aberrant and inactive population that is not comparable to murine VSEL cells.
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影响因子：
64.5
作者：
Kiel, MJ;Yilmaz, ÖH;Morrison, SJ
通讯作者：
Morrison, SJ
影响因子：
5.2
作者：
Henderson, JK;Draper, JS;Andrews, PW
通讯作者：
Andrews, PW
DOI：
10.1084/jem.20040440
发表时间：
2004-07-19
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Kögler G;Sensken S;Airey JA;Trapp T;Müschen M;Feldhahn N;Liedtke S;Sorg RV;Fischer J;Rosenbaum C;Greschat S;Knipper A;Bender J;Degistirici O;Gao J;Caplan AI;Colletti EJ;Almeida-Porada G;Müller HW;Zanjani E;Wernet P
通讯作者：
Wernet P
影响因子：
3
作者：
Han MV;Zmasek CM
通讯作者：
Zmasek CM
影响因子：
2.6
作者：
Alt, Rudiger;Wilhelm, Franziska;Cross, Michael
通讯作者：
Cross, Michael