A DELLA gene, RhGAI1, is a direct target of EIN3 and mediates ethylene-regulated rose petal cell expansion via repressing the expression of RhCesA2.
A DELLA gene, RhGAI1, is a direct target of EIN3 and mediates ethylene-regulated rose petal cell expansion via repressing the expression of RhCesA2.
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DELLA 基因 RhGAI1 是 EIN3 的直接靶标，通过抑制 RhCesA2 的表达来介导乙烯调节的玫瑰花瓣细胞扩增
DOI：
10.1093/jxb/ert296
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发表时间：
2013-11
影响因子：
6.9
通讯作者：
Gao J
中科院分区：
文献类型：
--
作者：
Luo J;Ma N;Pei H;Chen J;Li J;Gao J
乙烯在器官生长中起着重要作用。在拟南芥中，乙烯可以通过稳定della蛋白来抑制根的伸长。在以前的工作中，人们发现乙烯抑制了玫瑰花瓣细胞的扩张，并且乙烯诱导了5个della基因的单序列。然而，乙烯对della基因转录调控的机制尚不清楚。结果表明，在乙烯处理和ETR基因沉默的玫瑰花瓣中均能诱导RhGAI1的表达，而RhEIN3-3对拟南芥原生质体中的RhGAI1启动子活性有较强的诱导作用。此外，在凝胶迁移率改变分析(EMSA)中，RhEIN3-3可以直接与RhGAI1的启动子结合。在RhGAI1-Δ17过表达的拟南芥花瓣中，细胞的扩张受到抑制，而在RhGAI1沉默的玫瑰花瓣中，细胞的扩张受到促进。此外，在RhGAI1沉默的花瓣中，9个细胞扩张相关基因的表达发生了明显的变化，并且RhGAI1可以在EMSA中与RhCesA2的启动子结合。这些结果表明，RhGAI1在转录水平上受乙烯的调控，RhGAI1是RhEIN3-3的直接靶标。此外，RhGAI1被证明部分通过调节细胞扩张相关基因的表达而参与细胞扩张。此外，RhCesA2是RhGAI1的直接靶标。这项工作揭示了乙烯对RhGAI1的转录调节，并提供了更好的理解乙烯如何调节玫瑰花瓣扩张。
Ethylene plays an important role in organ growth. In Arabidopsis, ethylene can inhibit root elongation by stabilizing DELLA proteins. In previous work, it was found that ethylene suppressed cell expansion in rose petals, and five unisequences of DELLA genes are induced by ethylene. However, the mechanism of transcriptional regulation of DELLA genes by ethylene is still not clear. The results showed that the expression of RhGAI1 was induced in both ethylene-treated and ETR gene-silenced rose petals, and the promoter activity of RhGAI1 was strongly induced by RhEIN3-3 in Arabidopsis protoplasts. What is more, RhEIN3-3 could bind to the promoter of RhGAI1 directly in an electrophoretic mobility shift assay (EMSA). Cell expansion was suppressed in RhGAI1-Δ17-overexpressed Arabidopsis petals and promoted in RhGAI1-silenced rose petals. Moreover, in RhGAI1-silenced petals, the expression of nine cell expansion-related genes was clearly changed, and RhGAI1 can bind to the promoter of RhCesA2 in an EMSA. These results suggested that RhGAI1 was regulated by ethylene at the transcriptional level, and RhGAI1 was a direct target of RhEIN3-3. Also, RhGAI1 was shown to be involved in cell expansion partially through regulating the expression of cell expansion-related genes. Furthermore, RhCesA2 was a direct target of RhGAI1. This work uncovers the transcriptional regulation of RhGAI1 by ethylene and provides a better understanding of how ethylene regulates petal expansion in roses.
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影响因子：
7.4
作者：
Hauvermale, Amber L.;Ariizumi, Tohru;Steber, Camille M.
通讯作者：
Steber, Camille M.
影响因子：
11.6
作者：
Hirano, Ko;Asano, Kenji;Matsuoka, Makoto
通讯作者：
Matsuoka, Makoto
影响因子：
11.6
作者：
Hall, AE;Bleecker, AB
通讯作者：
Bleecker, AB
影响因子：
11.6
作者：
Hirsch, Sibylle;Kim, Jiyoung;Oldroyd, Giles E. D.
通讯作者：
Oldroyd, Giles E. D.
影响因子：
9.2
作者：
Achard, Patrick;Gusti, Andi;Genschik, Pascal
通讯作者：
Genschik, Pascal