Comparison of cytokine profiles in the aqueous humor of eyes with pseudoexfoliation syndrome and glaucoma.
Comparison of cytokine profiles in the aqueous humor of eyes with pseudoexfoliation syndrome and glaucoma.
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DOI：
10.1371/journal.pone.0182571
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Gerhardt C
中科院分区：
文献类型：
--
作者：
Garweg JG;Zandi S;Pfister IB;Skowronska M;Gerhardt C
比较继发性青光眼伴或不伴人工晶状体脱位的PEXS眼房水中细胞因子和趋化因子的分布。术中采集20例健康对照者和73例pex综合征患者的房水样本，pex综合征无其他局部或全身性疾病。后一组分为无并发症的早期PEX综合征（PEX, n = 33）、晚期PEX综合征合并青光眼（PEX, n = 30）和晚期PEX综合征合并人工晶状体脱位需要手术的患者（PEX, n = 10）。样品在-80°C保存后平行分析。使用Bio-Plex®多重微珠系统同时定量40种细胞因子的水平。组间资料比较采用Kruskal-Wallis检验（p≤0.01）。PEX和PEXG在所有40种细胞因子谱中均具有可比性，而pexl -眼睛的细胞因子谱显示除5种细胞因子（CXCL13、CCL27、IL-2、CCL3、CCL20; p≤0.01）外，其他细胞因子谱均较高。后一项发现表明在iol脱位的情况下存在非特异性炎症反应。各组中6种细胞因子浓度均低于检测限。pexl眼房水中85%可检测细胞因子的局部上调可能与疾病的进展或iol脱位时前后屏障的破坏有关。
To compare the cyto- and chemokine profiles in the aqueous humor of PEXS eyes in the absence or presence of secondary glaucoma with or without luxation of the intraocular lens (IOL). Samples of aqueous humor were collected intraoperatively from 20 healthy controls and from 73 eyes with PEX-syndrome, which was manifested in the absence of any other local or systemic desease. The latter group was sub-devided into eyes with an early form of PEX-syndrome in the absence of complications (PEX, n = 33), those with a late form of PEX-syndrome and glaucoma (PEXG, n = 30), and those with a late form of PEX-syndrome with luxation of the IOL that required surgery (PEXL, n = 10). The samples were analyzed in parallel after storage at -80°C. The levels of 40 cytokines were simultaneously quantified using the Bio-Plex® multiplex beads system. The inter-group data were statistically compared using the Kruskal-Wallis test (p ≤ 0.01). PEX and PEXG were comparable in their cytokine profiles for all 40 cytokines, whereas the cytokine profile in PEXL-eyes revealed higher levels of all but 5 cytokines (CXCL13, CCL27, IL-2, CCL3, CCL20; p ≤ 0.01). This latter finding is indicative of a non-specific inflammatory reaction in the context of IOL-luxation. The concentrations of 6 cytokines lay below the detection limit in all groups. The local up-regulation of 85% of the detectable cytokines in the aqueous humor of PEXL-eyes may be linked either with a progression of the disease or a breakdown of the antero-posterior barrier in the context of IOL-luxation.
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影响因子：
--
作者：
Schlötzer-Schrehardt, U;Küchle, M;Naumann, GOH
通讯作者：
Naumann, GOH
影响因子：
0.2
作者：
Djordjevic-Jocic, Jasmina;Zlatanovic, Gordana;Schlotzer-Schrehardt, Ursula
通讯作者：
Schlotzer-Schrehardt, Ursula
影响因子：
6
作者：
Zenkel, Matthias;Lewczuk, Piotr;Schloetzer-Schrehardt, Ursula
通讯作者：
Schloetzer-Schrehardt, Ursula
影响因子：
--
作者：
Schloetzer-Schrehardt, U.
通讯作者：
Schloetzer-Schrehardt, U.
影响因子：
4
作者：
Conway, RM;Schlötzer-Schrehardt, U;Naumann, GOH
通讯作者：
Naumann, GOH