Identification of C-Terminal Binding Protein 1 as a Novel NMDA Receptor Interactor.
Identification of C-Terminal Binding Protein 1 as a Novel NMDA Receptor Interactor.
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DOI：
10.1007/s11064-018-2633-5
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发表时间：
2019-06
影响因子：
4.4
通讯作者：
Stephenson FA
中科院分区：
文献类型：
--
作者：
Cousins SL;Stephenson FA
利用酵母双杂交技术筛选小鼠脑cDNA文库，发现了一个新的N-甲基-D-天冬氨酸神经递质受体相互作用蛋白。C-末端结合蛋白1（CtBP 1）与N-甲基D天冬氨酸受体亚基GluN 2A和GluN 2D的胞内C-末端区域相关，但不与GluN 1 -1a胞质C-末端区域相关。在使用一系列GluN 2A C-末端截短的酵母交配测定中，证明CtBP 1结合结构域定位于GluN 2A 1157-1382。GluN 2A结合结构域被鉴定为位于CtBP 1161 -224区域内。CtBP 1与哺乳动物细胞中表达的组装GluN 1/GluN 2A受体共免疫沉淀，并且还在成年小鼠脑的洗涤剂提取物中。CtBP 1与GluN 1/GluN 2A的共表达导致受体细胞表面表达显着降低。C-末端结合蛋白家族主要作为转录辅阻遏物起作用。然而，也已知它们调节细胞内膜运输机制。因此，本文报道的结果描述了CtBP 1在调节细胞表面N-甲基D天冬氨酸受体表达中的假定作用。
A new N-methyl D aspartate neurotransmitter receptor interacting protein has been identified by yeast two-hybrid screening of a mouse brain cDNA library. C-terminal binding protein 1 (CtBP1) was shown to associate with the intracellular C-terminal regions of the N-methyl D aspartate receptor subunits GluN2A and GluN2D but not with GluN1-1a cytoplasmic C-terminal region. In yeast mating assays using a series of GluN2A C-terminal truncations, it was demonstrated that the CtBP1 binding domain was localized to GluN2A 1157–1382. The GluN2A binding domain was identified to lie within the CtBP1 161–224 region. CtBP1 co-immunoprecipitated with assembled GluN1/GluN2A receptors expressed in mammalian cells and also, in detergent extracts of adult mouse brain. Co-expression of CtBP1 with GluN1/GluN2A resulted in a significant decrease in receptor cell surface expression. The family of C-terminal binding proteins function primarily as transcriptional co-repressors. However, they are also known to modulate intracellular membrane trafficking mechanisms. Thus the results reported herein describe a putative role for CtBP1 in the regulation of cell surface N-methyl D aspartate receptor expression.
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DOI：
10.1073/pnas.1002690107
发表时间：
2010-11-09
影响因子：
11.1
作者：
Bard, Lucie;Sainlos, Matthieu;Groc, Laurent
通讯作者：
Groc, Laurent
DOI：
10.1007/978-1-4939-7321-7_1
发表时间：
2017
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Hansen KB;Yi F;Perszyk RE;Menniti FS;Traynelis SF
通讯作者：
Traynelis SF
影响因子：
5.3
作者：
Verger, Alexis;Quinlan, Kate G. R.;Crossley, Merlin
通讯作者：
Crossley, Merlin
影响因子：
4.8
作者：
Riefler, GM;Firestein, BL
通讯作者：
Firestein, BL
Glun2亚基身份对NMDA受体功能的影响。
影响因子：
4.7
作者：
Wyllie DJ;Livesey MR;Hardingham GE
通讯作者：
Hardingham GE