Comparative analysis of intestinal bacteria among venom secretion and non-secrection snakes
Comparative analysis of intestinal bacteria among venom secretion and non-secrection snakes
复制标题
分泌毒液与非分泌毒液蛇肠道细菌的比较分析
DOI：
10.1038/s41598-019-42787-6
复制
发表时间：
2019-04
影响因子：
4.6
通讯作者：
Yeshi Yin
中科院分区：
文献类型：
--
作者：
Zuodong Qin;Siqi Wang;Dezhi Guo;Jialiang Zhu;Huahai Chen;Le Bai;Xiaofang Luo;Yeshi Yin
为了进一步研究分泌毒液蛇和不分泌毒液蛇的肠道细菌群落，并鉴定其生物标志物，从29条蛇（Deinagkistrodon 13条，Naja 26条，Ptyas mucosa 11条）中采集了50个肠道样品（大肠25个，小肠25个）。16S rDNA高通量测序结果显示，在这些样品中鉴定出29个细菌门，545个细菌属和1，725个OTU（操作分类单位）。OTU数和Ace，Chao，Shannon和Simpson指数在本研究中包括的三个品种的蛇之间非常相似。优势菌门为拟杆菌门、厚壁菌门、梭杆菌门和变形杆菌门。各样品在门水平上的相对丰度相近，小肠和大肠样品间的相对丰度差异不明显。但在属级水平上，分泌毒液的蛇Deinagkistrodon和Najac根据品种不同而聚在一起。分别鉴定出27属、24属和16属的核心微生物。有趣的是，哈夫尼亚菌属、肥胖杆菌属、普罗维登菌属和脲原体属的相对丰度在无毒液分泌的蛇中显著较高，无色杆菌属、鲸类杆菌属、无害梭菌群、梭杆菌属、毛梭菌属、副拟杆菌属和类杆菌属仅在有毒液分泌的蛇中检出。这些细菌在毒液分泌中的功能有待进一步研究，这些分泌毒液相关的属可能是提高毒液产量的有希望的靶点。
To further investigate the bacterial community and identify the bacterial biomarkers between venom secretion and non-venom secretion snakes, 50 intestinal samples (25 large intestine, 25 small intestine) were obtained from 29 snakes (13 gut samples fromDeinagkistrodon, 26 fromNajaand 11 fromPtyas mucosa). 16S rDNA high-throughput sequencing results showed that 29 bacterial phyla, 545 bacterial genera, and 1,725 OTUs (operational taxonomic units) were identified in these samples. OTU numbers and the Ace, Chao, Shannon, and Simpson indexes were very similar among the three breeds of snakes included in this study. TheBacteroidetes,Firmicutes,FusobacteriaandProteobacteriawere predominant bacterial phyla. The relative abundance at the phylum level among these samples was similar, and the difference between small and large intestinal samples was not obvious. However, at the genus level, venom secretion snakesDeinagkistrodonandNajaclustered together according to different breeds. 27, 24, and 16 genera were identified as core microbes forDeinagkistrodon,Naja, andPtyas mucosa, respectively. Interestingly, the relative abundances of generaHafnia_Obesumbacterium,Providencia, andUreaplasmawere found to be significantly higher in non-venom secretion snakes, and the generaAchromobacter,Cetobacterium,Clostridium innocuumgroup,Fusobacterium,Lachnoclostridium,Parabacteroides, andRomboutsiawere only detected in venom secretion snakes. The function of these bacteria in venom secretion needs to be further studied, and these venom secretion related genera may be the promising target to improve venom production.
登录
查看更多内容
肠道微生物群与肥胖及相关代谢紊乱之间的串扰
影响因子：
3.1
作者：
Xu Wen Ting;Nie Yong Zhan;Yang Zhen;Lu Nong Hua
通讯作者：
Lu Nong Hua
影响因子：
1.9
作者：
A. M. B. Amorim;J. S. Nascimento
通讯作者：
A. M. B. Amorim;J. S. Nascimento
DOI：
--
发表时间：
1984
期刊：
Zhonghua shen jing jing shen ke za zhi = Chinese journal of neurology and psychiatry
影响因子：
--
作者：
W. Hao
通讯作者：
W. Hao
DOI：
10.2174/187152412803760618
发表时间：
2012-11
影响因子：
--
作者：
A. Osipov;Y. Utkin
通讯作者：
A. Osipov;Y. Utkin
影响因子：
0.2
作者：
Sagar, Siddharth;Narasimhaswamy, Nagalakshmi;D'Souza, Jessica
通讯作者：
D'Souza, Jessica