Modulation of the Dental Pulp Stem Cell Secretory Profile by Hypoxia Induction Using Cobalt Chloride.
Modulation of the Dental Pulp Stem Cell Secretory Profile by Hypoxia Induction Using Cobalt Chloride.
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DOI：
10.3390/jpm11040247
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发表时间：
2021-03-30
影响因子：
--
通讯作者：
Patil S
中科院分区：
文献类型：
--
作者：
Bhandi S;Al Kahtani A;Mashyakhy M;Alsofi L;Maganur PC;Vishwanathaiah S;Testarelli L;Del Giudice A;Mehta D;Vyas N;Patil VR;Raj AT;Patil S
干细胞的作用是由它们的旁分泌介导的，旁分泌包括分泌型。可以使用各种方法来修饰干细胞的分泌谱。创造低氧环境是一种方法。本研究旨在证明氯化钴在牙髓干细胞（DPSC）培养中产生缺氧条件的影响，以及这种环境对其分泌特征的影响。从人恒牙中分离的DPSCs进行表征，并用不同浓度的CoCl 2处理，通过3-（4，5-二甲基噻唑-2-基）-2，5-二苯基四唑溴化物（MTT）测定来评估其活力，并通过细胞计数试剂盒（CCK）-8测定来评估其增殖。通过定量真实的时间聚合酶链反应（qRT-PCR）分析缺氧诱导因子1-α（HIF-1α）的基因表达水平以证明缺氧环境。采用流式细胞仪微球阵列检测细胞因子和生长因子。通过qRT-PCR分析转录因子OCT 4和SOX 2的基因表达水平，以了解CoCl 2对DPSC中的干性的影响。DPSC对MSC特异性标志物呈阳性。剂量高达20 µM的CoCl 2不会对细胞活力产生负面影响;在低剂量（5 µM）下，它会促进细胞存活。用10 μM CoCl 2处理显著增加了HIF-1α的基因表达。用10 µM CoCl 2处理的细胞显示出生长因子和细胞因子水平的变化。CoCl 2还增加了OCT 4和SOX 2的表达，这是DPSC干性的调节。CoCl 2处理诱导的低氧环境调节DPSC的分泌特征。
The action of stem cells is mediated by their paracrine secretions which comprise the secretory profile. Various approaches can be used to modify the secretory profile of stem cells. Creating a hypoxic environment is one method. The present study aims to demonstrate the influence of CoCl2 in generating hypoxic conditions in a dental pulp stem cell (DPSCs) culture, and the effect of this environment on their secretory profile. DPSCs that were isolated from human permanent teeth were characterized and treated with different concentrations of CoCl2 to assess their viability by an 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and proliferation by a cell counting kit (CCK)-8 assay. The gene expression level of hypoxia-inducible factor 1-alpha (HIF-1α) was analyzed by quantitative real time polymerase chain reaction (qRT-PCR) to demonstrate a hypoxic environment. Comparative evaluation of the growth factors and cytokines were done by cytometric bead array. Gene expression levels of transcription factors OCT4 and SOX2 were analyzed by qRT-PCR to understand the effect of CoCl2 on stemness in DPSCs. DPSCs were positive for MSC-specific markers. Doses of CoCl2, up to 20 µM, did not negatively affect cell viability; in low doses (5 µM), it promoted cell survival. Treatment with 10 µM of CoCl2 significantly augmented the genetic expression of HIF-1α. Cells treated with 10 µM of CoCl2 showed changes in the levels of growth factors and cytokines produced. It was very evident that CoCl2 also increased the expression of OCT4 and SOX2, which is the modulation of stemness of DPSCs. A CoCl2 treatment-induced hypoxic environment modulates the secretory profile of DPSCs.
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DOI：
10.1177/0271678x18776802
发表时间：
2018-08
期刊：
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism
影响因子：
--
作者：
Cunningham CJ;Redondo-Castro E;Allan SM
通讯作者：
Allan SM
影响因子：
3.8
作者：
Chen, Yijing;Zhao, Qi;Zhao, Wei
通讯作者：
Zhao, Wei
影响因子：
3.4
作者：
Chow, DC;Wenning, LA;Papoutsakis, ET
通讯作者：
Papoutsakis, ET
影响因子：
2.8
作者：
Hung, Shun-Pei;Ho, Jennifer H.;Lee, Oscar K.
通讯作者：
Lee, Oscar K.
影响因子：
5.6
作者：
Lee JH;Yoon YM;Lee SH
通讯作者：
Lee SH