Correlation between the coexpression of zinc finger and SCAN domain-containing protein 31 and transcriptional activator with PDZ-binding motif and prognosis in hepatocellular carcinoma.
Correlation between the coexpression of zinc finger and SCAN domain-containing protein 31 and transcriptional activator with PDZ-binding motif and prognosis in hepatocellular carcinoma.
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锌指与含SCAN结构域蛋白31及PDZ结合基序转录激活因子的共表达与肝细胞癌预后的相关性
DOI：
10.21037/atm-20-6373
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发表时间：
2020-10
影响因子：
--
通讯作者：
Xiao H
中科院分区：
文献类型：
--
作者：
Liu Q;Shi Z;Liu X;Xiao H
背景转录辅激活因子与PDZ结合基序（TAZ）调控多种生物学过程，并已被发现与肝细胞癌（HCC）有关。然而，TAZ敲低后下游的共同信号传导途径也可能很重要。方法在肝癌细胞系中敲除TAZ，并分析其潜在的靶基因。观察到锌指和含SCAN结构域蛋白31（ZSCAN 31）的表达减少。评估ZSCAN 31表达的差异，并确定其对接受手术切除的HCC患者生存率的影响。结果ZSCAN 31在肝癌组织中过表达，与肝癌患者手术切除后的低总生存率相关。对83例在我院接受手术切除的HCC患者的组织样本进行分析，得出了类似的结果。ZSCAN 31高表达与肿瘤大小显著相关。TAZ和ZSCAN 31的高表达水平与较差的OS相关。ZSCAN 31表达和TAZ表达之间呈正相关，并且使用HCC细胞系通过免疫共沉淀（Co-IP）测定证实了ZSCAN 31和TAZ的蛋白结合。结论ZSCAN 31与TAZ在肝癌细胞中的表达相关，靶向ZSCAN 31和TAZ可能成为肝癌治疗的新途径。
Background Transcriptional coactivator with PDZ binding motif (TAZ) regulates multiple biological processes and has been found to be related to hepatocellular carcinoma (HCC). However, common signaling pathways downstream after TAZ knockdown may also be important. Methods TAZ was knocked down in an HCC cell line, and its potential target genes were analyzed. A decrease in the expression of zinc finger and SCAN domain-containing protein 31 (ZSCAN31) was observed. The difference in ZSCAN31 expression was evaluated, and its effect on survival in HCC patients who received surgical resection was determined. Results ZSCAN31 was over-expressed in HCC tissues and was associated with low overall survival (OS) in HCC patients after surgical resection. Analysis of tissue samples from 83 HCC patients who underwent surgical resection in our hospital produced similar results. High ZSCAN31 expression was significantly associated with tumor size. High expression levels of both TAZ and ZSCAN31 were related to poor OS. A positive correlation was identified between ZSCAN31 expression and TAZ expression, and the protein binding of ZSCAN31 and TAZ was confirmed by co-immunoprecipitation (Co-IP) assay using an HCC cell line. Conclusions ZSCAN31 is associated with TAZ expression in HCC cells, and the targeting of ZSCAN31 and TAZ may represent a novel therapeutic approach in HCC.
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影响因子：
8.8
作者：
通讯作者：
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DOI：
10.1038/nrd4161
发表时间：
2014-01
期刊：
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影响因子：
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影响因子：
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作者：
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通讯作者：
Guan, Kun-Liang
影响因子：
11.4
作者：
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通讯作者：
Yaffe, MB