Constructing bioactive peptides with pH-dependent activities.
Constructing bioactive peptides with pH-dependent activities.
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DOI：
10.1016/j.peptides.2009.05.009
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发表时间：
2009-08
期刊：
影响因子：
3
通讯作者：
Liang JF
中科院分区：
文献类型：
--
作者：
Tu Z;Volk M;Shah K;Clerkin K;Liang JF
许多生物活性肽的特点是富含精氨酸和赖氨酸。在这项研究中，裂解肽中的赖氨酸和精氨酸残基被组氨酸选择性取代。尽管所得含组氨酸的裂解肽活性降低，但它们确实显示出pH依赖性细胞毒性。当溶液pH从7.4变为5.5时，构建的含组氨酸裂解肽的活性增加了2 - 8倍。更重要的是，这些含组氨酸的肽通过引起细胞裂解，保持了与其母体肽相同的细胞杀伤机制。含组氨酸肽的活性和pH敏感性均可通过调整组氨酸取代的数量和位置来调节。这项研究提出了一种通用策略，用于创建具有所需pH敏感性的生物活性肽，以满足癌症治疗等各种应用的需求。
Many bioactive peptides are featured by their arginine and lysine rich contents. In this study, lysine and arginine residues in lytic peptides were selectively replaced by histidines. Although resulted histidine-containing lytic peptides had decreased activity, they did show pH-dependent cytotoxicity. The activity of the constructed histidine-containing lytic peptides increased 2 ~ 8 times as the solution pH changed from of 7.4 to 5.5. More importantly, these histidine-containing peptides maintain the same cell killing mechanism as their parent peptides by causing cell lysis. Both the activity and pH-sensitivity of histidine-containing peptides are tunable by adjusting histidine substitution numbers and positions. This study has presented a general strategy to create bioactive peptides with desired pH-sensitivity to meet the needs of various applications such as cancer treatments.
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DOI：
10.1016/j.bbrc.2007.04.132
发表时间：
2007-06-22
影响因子：
3.1
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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影响因子：
3
作者：
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通讯作者：
Wada, Keiji
影响因子：
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