Thermoresponsive hydrogel as a delivery scaffold for transfected rat mesenchymal stem cells.
Thermoresponsive hydrogel as a delivery scaffold for transfected rat mesenchymal stem cells.
复制标题
DOI：
10.1021/mp100094k
复制
发表时间：
2010-08-02
影响因子：
4.9
通讯作者：
Kim SW
中科院分区：
文献类型：
--
作者：
Borden BA;Yockman J;Kim SW
干细胞作为一种治疗剂的概念已经获得了动力。这些细胞的常见给药方式是直接注射到靶组织中。这可能导致许多细胞由于注射部位的回流而丢失，从而导致植入细胞的局部丢失。PoligoGel是一种无毒水凝胶，LCST接近体温。它还显示对多种细胞类型无毒，并且在大鼠间充质干细胞的情况下，不会通过诱导分化或限制从凝胶中取出后随后分化的潜力来改变其分化能力。将细胞包埋在PoligoGel中也不会干扰细胞在用质粒DNA转染后递送治疗性生长因子的能力。在这里，温度响应性水凝胶PoligoGel显示出具有作为用于将细胞保留在注射部位的支架的潜力，从而在植入后减轻细胞从靶部位迁移或洗涤。
The concept of stem cells as a therapeutic agent has been gaining momentum. A common mode of administration of these cells is by direct injection into the target tissue. This can result in many of the cells being lost due to reflux from the injection site leading to a local loss of implanted cells. PoligoGel is a non-toxic hydrogel with an LCST near body temperature. It is also shown to be non-toxic to multiple cell types, and in the case of rat mesenchymal stem cells does not alter their differentiative capacity, either by inducing differentiation, or limiting the potential for subsequent differentiation after removal from the gel. Embedding cells in PoligoGel also does not interfere with the cells ability to delivery therapeutic growth factors post transfection with plasmid DNA. Here a thermoresponsive hydrogel, PoligoGel, is shown to have potential to act as a scaffold for the retention of cells at an injection site, mitigating migration or washing of the cells away from the target site after implantation.
登录
查看更多内容
影响因子：
37.8
作者：
Itzhaki-Alfia, Ayelet;Leor, Jonathan;Barbash, Israel M.
通讯作者：
Barbash, Israel M.
影响因子：
2.6
作者：
Wagner, W;Wein, F;Ho, AD
通讯作者：
Ho, AD
影响因子：
2.4
作者：
Nicodemus, G. D.;Bryant, S. J.
通讯作者：
Bryant, S. J.
影响因子：
64.8
作者：
Jeong, B;Bae, YH;Kim, SW
通讯作者：
Kim, SW
影响因子：
14
作者：
Nguyen, KT;West, JL
通讯作者：
West, JL