Membrane-impermeant crosslinking reagents: probes of the structure and dynamics of membrane proteins
Membrane-impermeant crosslinking reagents: probes of the structure and dynamics of membrane proteins
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膜不渗透的交联试剂：膜蛋白结构和动力学的探针
DOI：
10.1021/ar00156a001
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发表时间：
1988
影响因子：
18.3
通讯作者：
J. V. Staros
中科院分区：
文献类型：
--
作者：
J. V. Staros
每一个活细胞都被一个由脂质和蛋白质以及少量碳水化合物组成的质膜所包围。1，2脂质形成双层，作为无机离子和亲水性分子进出细胞的屏障。碳水化合物，大部分是糖蛋白或糖脂的组成部分，似乎在细胞-细胞识别等过程中起作用。然而，对于跨膜过程，即无机离子或亲水性有机分子选择性地移入或移出细胞或激素与细胞表面受体结合的跨膜信号传导，蛋白质是关键角色。执行跨膜功能的蛋白质通常在结构上是跨膜的，并且特定蛋白质的每个拷贝在膜中具有相同的取向。[2]早期对许多膜蛋白的研究表明，这些蛋白是寡聚的，或者由不同的亚基组成，如Na+，K+-ATP酶，[3]或者由两个或多个拷贝的单个亚基组成，如红细胞的阴离子交换通道。[4]其他研究表明，许多跨膜蛋白可以动态地与细胞质中的纤维状聚合蛋白结构相结合，这些结构统称为细胞骨架或膜骨架。詹姆斯·斯塔罗斯生于1947年，大约20年后开始了他的科学生涯，当时他还是达特茅斯学院的一名本科生，他加入了大卫勒马尔的研究小组，研究物理有机化学中的一个问题。从达特茅斯毕业后，他作为一名NSF毕业生来到耶鲁大学。他获得了博士学位。1974年毕业于耶鲁大学分子生物物理学和生物化学专业，师从弗雷德里克理查兹。接下来，他搬到了哈佛大学化学系，在那里他与杰里米·诺尔斯一起成为海伦·海·惠特尼的博士后研究员。1978年，他搬到了范德比尔特大学，在那里他目前是生物化学教授，并彻底享受生活在化学和生物学的接口。
Every living cell is bounded by a plasma membrane composed of lipids and proteins, plus a small amount of carbohydrate. 1, 2 The lipids form a bilayer that acts as a barrier to the passage of inorganic ions and hy-drophilic molecules into or out of the cell. The carbohydrate, which for the most part occurs as a component of glycoproteins or glycolipids, appearsto function in processes such as cell-cell recognition. However, for transmembrane processes, ie, the selective movement of inorganic ions or of hydrophilic organic molecules into or out of the cell or the transmembrane signaling of the binding of a hormone to a receptor on the cell surface, proteins are the key players. Proteins that carry out transmembrane functions are generally transmembrane in structure, and every copy of a particular protein has the same orientation in the membrane. 2 Early studies of a number of membrane proteins had suggested that these proteins were oli-gomeric, either made up of different subunits, as in the case of the Na+, K+-ATPase, 3 or made up of two or more copies of a single subunit, as in the case of the anion-exchange channel of the erythrocyte. 4 Other studies had demonstrated that many transmembrane proteins can be dynamically associated with fibrous, polymeric protein structures in the cytoplasm of the cell, collectively known as the cytoskeleton or mem-brane skeleton.James V. Staros was born In 1947 and began his scientific career some 20 years later, when, as an undergraduate at Dartmouth College, he joined the research group of David Lemal to work on a problem In physical organic chemistry. After graduating from Dartmouth, he moved to Yale University as an NSFGraduate FeHow. He received the Ph. D. from Yale In 1974 in molec-ular biophysics and biochemistry, having worked under the tutelage of Frederic Richards. Next he moved to the Department of Chemistry at Harvard University, where he was a Helen Hay Whitney Postdoctoral Fellow with Jeremy Knowles. In 1978, he moved to Vanderbilt University, where he is presently Professor of Biochemistry and thoroughly enjoying life at the interface of chemistry and biology.
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影响因子：
3.6
作者：
Cohen,CM
通讯作者：
Cohen,CM
人红细胞中的阴离子载体以二聚体形式存在。
DOI：
--
发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Cuppoletti,J;Goldinger,J;Kang,B;Jo,I;Berenski,C;Jung,CY
通讯作者：
Jung,CY
影响因子：
2.9
作者：
STAROS, JV;WRIGHT, RW;SWINGLE, DM
通讯作者：
SWINGLE, DM
影响因子：
2.9
作者：
Packard,B;Edidin,M;Komoriya,A
通讯作者：
Komoriya,A
与 A431 细胞膜囊泡中表皮生长因子受体相关的蛋白激酶的亲和标记。
DOI：
--
发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Buhrow,SA;Cohen,S;Staros,JV
通讯作者：
Staros,JV