Increase methylmercury accumulation in Arabidopsis thaliana expressing bacterial broad-spectrum mercury transporter MerE.
Increase methylmercury accumulation in Arabidopsis thaliana expressing bacterial broad-spectrum mercury transporter MerE.
复制标题
增加表达细菌广谱汞转运蛋白 MerE 的拟南芥中甲基汞的积累。
DOI：
10.1186/2191-0855-3-52
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发表时间：
2013-09-03
期刊：
影响因子：
3.7
通讯作者：
Kiyono M
中科院分区：
文献类型：
--
作者：
Sone Y;Nakamura R;Pan-Hou H;Sato MH;Itoh T;Kiyono M
来自Tn21 mer操纵子的细菌merE基因编码一个广谱汞转运体，该转运体控制甲基汞和汞离子在细菌细胞质膜上的转运，该基因是提高甲基汞植物修复效率的潜在分子工具。构建了表达MerE的转基因拟南芥，并评价了MerE表达对甲基汞积累的影响。在拟南芥悬浮培养细胞中检测瞬时表达gfp标记的MerE的亚细胞定位。发现GFP-MerE定位于质膜和细胞质溶胶。表达MerE的转基因拟南芥比野生型拟南芥积累了更多的甲基汞和汞离子。与野生型拟南芥相比，表达MerE的转基因植株对汞离子的抗性显著增强，但仅表现出对甲基汞的抗性。这些结果表明，细菌汞转运体MerE的表达促进了甲基汞在转基因拟南芥中的转运和积累，这可能是一种促进甲基汞污染植物修复的有效方法。
The bacterial merE gene derived from the Tn21 mer operon encodes a broad-spectrum mercury transporter that governs the transport of methylmercury and mercuric ions across bacterial cytoplasmic membranes, and this gene is a potential molecular tool for improving the efficiency of methylmercury phytoremediation. A transgenic Arabidopsis engineered to express MerE was constructed and the impact of expression of MerE on methylmercury accumulation was evaluated. The subcellular localization of transiently expressed GFP-tagged MerE was examined in Arabidopsis suspension-cultured cells. The GFP-MerE was found to localize to the plasma membrane and cytosol. The transgenic Arabidopsis expressing MerE accumulated significantly more methymercury and mercuric ions into plants than the wild-type Arabidopsis did. The transgenic plants expressing MerE was significantly more resistant to mercuric ions, but only showed more resistant to methylmercury compared with the wild type Arabidopsis. These results demonstrated that expression of the bacterial mercury transporter MerE promoted the transport and accumulation of methylmercury in transgenic Arabidopsis, which may be a useful method for improving plants to facilitate the phytoremediation of methylmercury pollution.
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影响因子：
13.8
作者：
Lyyra, Satu;Meagher, Richard B.;Merkle, Scott A.
通讯作者：
Merkle, Scott A.
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
DOI：
10.1073/pnas.88.3.834
发表时间：
1991-02-01
影响因子：
11.1
作者：
MATSUOKA, K;NAKAMURA, K
通讯作者：
NAKAMURA, K
影响因子：
4.1
作者：
Heaton, ACP;Rugh, CL;Meagher, RB
通讯作者：
Meagher, RB
影响因子：
3.9
作者：
Kiyono, Masako;Oka, Yumiko;Pan-Hou, Hidemitsu
通讯作者：
Pan-Hou, Hidemitsu