Krüppel-Like Factor 10 participates in cervical cancer immunoediting through transcriptional regulation of Pregnancy-Specific Beta-1 Glycoproteins.
Krüppel-Like Factor 10 participates in cervical cancer immunoediting through transcriptional regulation of Pregnancy-Specific Beta-1 Glycoproteins.
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DOI：
10.1038/s41598-018-27711-8
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发表时间：
2018-06-21
影响因子：
4.6
通讯作者：
Salcedo M
中科院分区：
文献类型：
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作者：
Marrero-Rodríguez D;Taniguchi-Ponciano K;Subramaniam M;Hawse JR;Pitel KS;Arreola-De la Cruz H;Huerta-Padilla V;Ponce-Navarrete G;Figueroa-Corona MDP;Gomez-Virgilio L;Martinez-Cuevas TI;Mendoza-Rodriguez M;Rodriguez-Esquivel M;Romero-Morelos P;Ramirez-Salcedo J;Baudis M;Meraz-Rios M;Jimenez-Vega F;Salcedo M
宫颈癌(CC)与免疫系统平衡的改变有关，这主要是由于Th1向Th2的转变和Th17/Treg细胞的失衡。在电子DNA拷贝数分析中，我们发现~20%的CC样本分别表现出8q22.3和19q13.31的增益，即基因组中编码KLF10和PSG基因的区域。基因表达研究表明，癌组织中KLF10mRNA的表达无明显变化，而PSG2和−5基因的表达水平分别为正常对照组的1.76倍和3.97倍。SiRNA和芯片实验证明，KLF10通过调节IL6、IL25、PSG2和PSG5基因参与免疫应答。利用KLF10−/−小鼠的宫颈组织，我们发现PSG17、−21、−23和IL 11的表达下调。这些结果表明，KLF10可能在调节宫颈癌免疫系统反应基因等方面发挥作用。KLF10和PSG拷贝数变异和mRNA表达水平的改变可能是CC新的分子标记。
Cervical cancer (CC) is associated with alterations in immune system balance, which is primarily due to a shift from Th1 to Th2 and the unbalance of Th17/Treg cells. Using in silico DNA copy number analysis, we have demonstrated that ~20% of CC samples exhibit gain of 8q22.3 and 19q13.31; the regions of the genome that encodes the KLF10 and PSG genes, respectively. Gene expression studies demonstrated that there were no alterations in KLF10 mRNA expression, whilst the PSG2 and −5 genes were up-regulated by 1.76 and 3.97-fold respectively in CC compared to normal tissue controls. siRNA and ChIP experiments in SiHa cells have demonstrated that KLF10 participates in immune response through regulation of IL6, IL25 and PSG2 and PSG5 genes. Using cervical tissues from KLF10−/− mice, we have identified down-regulation of PSG17, −21 and −23 and IL11. These results suggest that KLF10 may regulate immune system response genes in cervical cancer among other functions. KLF10 and PSG copy number variations and alterations in mRNA expression levels could represent novel molecular markers in CC.
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影响因子：
7.3
作者：
Khedkar SA;Sun X;Rigby AC;Feinberg MW
通讯作者：
Feinberg MW
影响因子：
3.6
作者：
Diehl, S;Rincón, M
通讯作者：
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3.2
作者：
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Lepique, Ana Paula
影响因子：
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作者：
Tjiong, MY;van der Vange, N;Out, TA
通讯作者：
Out, TA
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DOI：
10.1161/atvbaha.112.301049
发表时间：
2013-07
期刊：
Arteriosclerosis, thrombosis, and vascular biology
影响因子：
--
作者：
Ju X;Ijaz T;Sun H;Ray S;Lejeune W;Lee C;Recinos A 3rd;Guo DC;Milewicz DM;Tilton RG;Brasier AR
通讯作者：
Brasier AR