Effect of hyperketonemia (Acetoacetate) on nuclear factor-κB and p38 mitogen-activated protein kinase activation mediated intercellular adhesion molecule 1 upregulation in endothelial cells.
Effect of hyperketonemia (Acetoacetate) on nuclear factor-κB and p38 mitogen-activated protein kinase activation mediated intercellular adhesion molecule 1 upregulation in endothelial cells.
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高酮血症（乙酰乙酸）对核因子-κB 和 p38 丝裂原激活蛋白激酶激活介导的内皮细胞中细胞间粘附分子 1 上调的影响。
DOI：
10.1089/met.2014.0101
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发表时间：
2015
影响因子：
2.1
通讯作者：
Jain,SushilK
中科院分区：
文献类型：
--
作者：
Rains,JustinL;Jain,SushilK
Background:Hyperketonemia is a pathological condition observed in patients with type 1 diabetes and ketosis-prone diabetes (KPD), which results in increased blood levels of acetoacetate (AA) and β-hydroxybutyrate (BHB). Frequent episodes of hyperketonemia are associated with a higher incidence of vascular disease. We examined the hypothesis that hyperketonemia activates the nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways that regulate intercellular adhesion molecule 1 (ICAM-1) expression in endothelial cells.Methods:Human umbilical vein endothelial cells (HUVECs) were cultured with AA (0–8 mM) or BHB (0–10 mM) for 0–24 hr. Western blotting was used to determine NF-κB activation in whole-cell lysates. ICAM-1 expression was measured using flow cytometry.Results:Results show a 2.4-fold increase in NF-κB activation in cells treated with 8 mM AA compared to the control. BHB had little or no effect on NF-κB activation. Pretreatment with a reactive oxygen species (ROS) inhibitor [N-acetyl-l-cysteine (NAC)] reduced NF-κB to near-control levels. The expression of AA-induced ICAM-1 was significantly reduced when cells were pretreated with either NAC or p38 MAPK inhibitor.Conclusions:These results suggest that NF-κB and p38 MAPK mediate upregulation of ICAM-1 expression in endothelial cells exposed to elevated levels of AA, which may contribute to the development of vascular disease in diabetes.
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DOI：
10.1016/0309-1651(80)90064-8
发表时间：
1980
期刊：
Cell biology international reports
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1986
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1982
期刊：
The Journal of investigative dermatology
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Uitto,J;Ryhänen,L;Abraham,PA;Perejda,AJ
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Perejda,AJ
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The Journal of investigative dermatology
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R. Sandhaus
影响因子：
6.5
作者：
SZENDROI, M;MEIMON, G;HORNEBECK, W
通讯作者：
HORNEBECK, W