The dinoflagellates Durinskia baltica and Kryptoperidinium foliaceum retain functionally overlapping mitochondria from two evolutionarily distinct lineages.
The dinoflagellates Durinskia baltica and Kryptoperidinium foliaceum retain functionally overlapping mitochondria from two evolutionarily distinct lineages.
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DOI：
10.1186/1471-2148-7-172
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发表时间：
2007-09-24
影响因子：
3.4
通讯作者：
Keeling PJ
中科院分区：
文献类型：
--
作者：
Imanian B;Keeling PJ
海藻、波罗的海海藻和叶海鞘藻类的区别在于含有一种来源于硅藻内共生体的第三级质体。硅藻与宿主细胞周期完全整合，在结构上发生了如此大的变化，以至于很难识别它是硅藻，但它保留了一些通常在第三级和第二级内共生体中失去的特征，最明显的是线粒体。据报道，甲藻宿主还保留了线粒体样结构，使这些细胞在保留两个进化上截然不同的线粒体方面独一无二。这种冗余性提出了一个问题，即这些细胞器之间是否有任何共同的功能或具有分散的功能。我们发现寄主和内共生体线粒体基因组都编码电子传递蛋白的基因。我们已经鉴定了内共生体线粒体祖先的细胞色素C氧化酶1(Cox1)、细胞色素氧化酶2(COX2)、细胞色素氧化酶3(Cox3)、细胞色素b(Cob)和核糖体RNA大亚基(LSUrRNA)，以及宿主线粒体祖先的Cox1和Cob。我们发现所有的基因都是转录的，那些归因于宿主线粒体基因组的基因在RNA水平上被广泛编辑，正如甲藻线粒体编码基因所预期的那样。我们还发现了宿主线粒体基因广泛重组的证据，重组产物也被转录，就像预期的甲藻一样。波罗的海云杉和叶海棠保留了进化上不同谱系的两个线粒体，这些细胞器的功能至少部分重叠，因为它们都表达电子传递蛋白质的基因。
The dinoflagellates Durinskia baltica and Kryptoperidinium foliaceum are distinguished by the presence of a tertiary plastid derived from a diatom endosymbiont. The diatom is fully integrated with the host cell cycle and is so altered in structure as to be difficult to recognize it as a diatom, and yet it retains a number of features normally lost in tertiary and secondary endosymbionts, most notably mitochondria. The dinoflagellate host is also reported to retain mitochondrion-like structures, making these cells unique in retaining two evolutionarily distinct mitochondria. This redundancy raises the question of whether the organelles share any functions in common or have distributed functions between them. We show that both host and endosymbiont mitochondrial genomes encode genes for electron transport proteins. We have characterized cytochrome c oxidase 1 (cox1), cytochrome oxidase 2 (cox2), cytochrome oxidase 3 (cox3), cytochrome b (cob), and large subunit of ribosomal RNA (LSUrRNA) of endosymbiont mitochondrial ancestry, and cox1 and cob of host mitochondrial ancestry. We show that all genes are transcribed and that those ascribed to the host mitochondrial genome are extensively edited at the RNA level, as expected for a dinoflagellate mitochondrion-encoded gene. We also found evidence for extensive recombination in the host mitochondrial genes and that recombination products are also transcribed, as expected for a dinoflagellate. Durinskia baltica and K. foliaceum retain two mitochondria from evolutionarily distinct lineages, and the functions of these organelles are at least partially overlapping, since both express genes for proteins in electron transport.
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影响因子：
2.2
作者：
Imanian, Behzad;Carpenter, Kevin J.;Keeling, Patrick J.
通讯作者：
Keeling, Patrick J.
影响因子：
3.9
作者：
Norman, JE;Gray, MW
通讯作者：
Gray, MW
影响因子：
2.9
作者：
DODGE, JD
通讯作者：
DODGE, JD
影响因子：
5.6
作者：
Lin, SJ;Zhang, HA;Gray, MW
通讯作者：
Gray, MW
影响因子：
3
作者：
CHESNICK, JM;COX, ER
通讯作者：
COX, ER