Effect of nicotine and porphyromonas gingivalis lipopolysaccharide on endothelial cells in vitro.
Effect of nicotine and porphyromonas gingivalis lipopolysaccharide on endothelial cells in vitro.
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DOI：
10.1371/journal.pone.0096942
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Rausch-Fan X
中科院分区：
文献类型：
--
作者：
An N;Andrukhov O;Tang Y;Falkensammer F;Bantleon HP;Ouyang X;Rausch-Fan X
吸烟被认为是牙周病和心血管疾病（CVD）的重要危险因素。内皮细胞在这两种疾病的进展中起重要作用。本研究在体外研究了尼古丁对牙龈卟啉单胞菌脂多糖刺激人脐静脉内皮细胞（HUVECs）功能特性的影响。不同浓度的尼古丁（10µM-10 mM）和/或P. gingivalis LPS刺激huvec。在基因和蛋白水平上检测细胞间粘附分子-1、血管细胞粘附分子-1、e-选择素、单核细胞趋化蛋白1和白细胞介素-8的表达水平。细胞增殖/活力，凋亡和迁移也进行了研究。浓度为10 mM的尼古丁刺激4 h后显著降低了牙龈卟啉卟啉多糖诱导的所有研究蛋白的表达，而较低浓度的尼古丁对有无牙龈卟啉卟啉多糖的蛋白表达无显著影响。10-mM尼古丁对HUVECs的增殖/活力也有显著抑制作用，但较低浓度的尼古丁对HUVECs的增殖/活力无明显抑制作用。浓度为1 ~ 10 mM的尼古丁可显著降低HUVECs的迁移。与吸烟者血清中尼古丁浓度相似的浓度对HUVECs的功能特性无显著影响。然而，高浓度的尼古丁，类似于在吸烟者的口腔中观察到的，抑制了huvec的炎症反应。尼古丁的这种作用可能与降低吸烟牙周炎患者的牙龈出血指数有关。
Smoking is considered a significant risk factor for both periodontal disease and cardiovascular disease (CVD). Endothelial cells play an important role in the progression of both diseases. In the present study, we investigated in vitro the impact of nicotine on functional properties of human umbilical vein endothelial cells (HUVECs) stimulated with lipopolysaccharide (LPS) of periodontal pathogen Porphyromonas gingivalis. HUVECs were stimulated with different concentrations of nicotine (10 µM-10 mM) and/or P. gingivalis LPS. Expression levels of intercellular adhesion molecule-1, vascular cell adhesion molecule-1, E-selectin, monocyte chemoattractant protein 1, and interleukin-8 were measured on both gene and protein levels. Cell proliferation/viability, apoptosis, and migration were also investigated. Nicotine at a concentration of 10 mM significantly decreased P. gingivalis LPS-induced expression of all investigated proteins after 4 h stimulation, while lower nicotine concentrations had no significant effect on protein expression with or without P. gingivalis LPS. Proliferation/viability of HUVECs was also significantly inhibited by 10-mM nicotine but not by lower concentrations. Migration of HUVECs was significantly decreased by nicotine at concentrations of 1–10 mM. Nicotine at a concentration similar to that observed in the serum of smokers had no significant effect on the functional properties of HUVECs. However, high concentrations of nicotine, similar to that observed in the oral cavity of smokers, inhibited the inflammatory response of HUVECs. This effect of nicotine might be associated with decreased gingival bleeding indices in smoking periodontitis patients.
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影响因子：
3.5
作者：
MOUGHAL, NA;ADONOGIANAKI, E;KINANE, DF
通讯作者：
KINANE, DF
影响因子：
3.5
作者：
GEMMELL, E;WALSH, LJ;SEYMOUR, GJ
通讯作者：
SEYMOUR, GJ
影响因子：
6.1
作者：
Li, XW;Wang, H
通讯作者：
Wang, H
影响因子：
5.3
作者：
Nakamura, Naokazu;Yoshida, Masayuki;Iwai, Takehisa
通讯作者：
Iwai, Takehisa
影响因子：
3.1
作者：
Nassar, H;Chou, HH;Genco, CA
通讯作者：
Genco, CA