Responses of gut microbiota to diet composition and weight loss in lean and obese mice.
Responses of gut microbiota to diet composition and weight loss in lean and obese mice.
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DOI：
10.1038/oby.2011.111
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发表时间：
2012-04
期刊：
影响因子：
--
通讯作者：
Leibel RL
中科院分区：
文献类型：
--
作者：
Ravussin Y;Koren O;Spor A;LeDuc C;Gutman R;Stombaugh J;Knight R;Ley RE;Leibel RL
维持降低的体重伴随着能量消耗的降低，超出了由体重和组成减少所引起的能量消耗，以及进食欲望的增加。这些影响似乎是由于-部分-由于身体脂肪的损失，循环瘦素浓度降低。肠道微生物群与体重的调节有关。已经在人类和小鼠中研究了体重减轻对肠道微生物群定性方面的影响，但这些研究受到影响微生物群落组成的饮食组成的同时变化的混淆。我们研究了20%的体重减轻对高脂饮食（HFD）的饮食诱导的肥胖（DIO：60%卡路里脂肪）小鼠的微生物群的影响。体重减轻的DIO（DIO-WR）小鼠具有与喂食对照饮食（10%卡路里脂肪）的对照（CON）随意（AL）喂食小鼠相同的体重和组成，允许直接比较饮食和体重扰动效应。通过焦磷酸测序来自处死动物盲肠的16 S rRNA基因来评估微生物群落组成。饮食组成对微生物群的多样性和组成有很大的影响。在所有小鼠的皮下白色脂肪组织中，微生物群的特定成员的相对丰度与循环瘦素浓度和炎症标志物的基因表达水平相关。总之，这些结果表明，宿主肥胖和饮食组成都影响微生物群组成，可能是通过瘦素介导的粘液产生和/或改变肠道栖息地的炎症过程的调节。
Maintenance of a reduced body weight is accompanied by a decrease in energy expenditure beyond that accounted for by reduced body mass and composition, as well as by an increased drive to eat. These effects appear to be due— in part—to reductions in circulating leptin concentrations due to loss of body fat. Gut microbiota have been implicated in the regulation of body weight. The effects of weight loss on qualitative aspects of gut microbiota have been studied in humans and mice, but these studies have been confounded by concurrent changes in diet composition, which influence microbial community composition. We studied the impact of 20% weight loss on the microbiota of diet-induced obese (DIO: 60% calories fat) mice on a high-fat diet (HFD). Weight-reduced DIO (DIO-WR) mice had the same body weight and composition as control (CON) adlibitum (AL) fed mice being fed a control diet (10% calories fat), allowing a direct comparison of diet and weight-perturbation effects. Microbial community composition was assessed by pyrosequencing 16S rRNA genes derived from the ceca of sacrificed animals. There was a strong effect of diet composition on the diversity and composition of the microbiota. The relative abundance of specific members of the microbiota was correlated with circulating leptin concentrations and gene expression levels of inflammation markers in subcutaneous white adipose tissue in all mice. Together, these results suggest that both host adiposity and diet composition impact microbiota composition, possibly through leptin-mediated regulation of mucus production and/or inflammatory processes that alter the gut habitat.
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影响因子：
120.7
作者：
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通讯作者：
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DOI：
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发表时间：
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DOI：
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发表时间：
2008-11-18
影响因子：
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作者：
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DOI：
10.1152/ajpgi.00091.2007
发表时间：
2007-07-01
影响因子：
4.5
作者：
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